UNC13A Antibody
Catalog Number: LS-C752054-100
100 µg / $359.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human UNC13A Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G88687-20
Catalog Number: LS-G88687
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human UNC13A (NM_001080421) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1032 | Munc13-1 | Unc-13 homolog A (C. elegans) | UNC13A | Protein unc-13 homolog A
- Origin Species
- Human
- NCBI GenBank
- AB028955
- SwissProt
- Q9UPW8
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 202.12 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGGGTCTIS VQVRVCTGGN LLGGRRRPLS PCNEEVWGEA PLPGARPFNA PPARSAPAPS61GVPGLRLPAP HQRHSRPGRL RPTCELGDMS LLCVGVKKAK FDGAQEKFNT YVTLKVQNVK121STTIAVRGSQ PSWEQDFMFE INRLDLGLTV EVWNKGLIWD TMVGTVWIPL RTIRQSNEEG181PGEWLTLDSQ VIMADSEICG TKDPTFHRIL LDTRFELPLD IPEEEARYWA KKLEQLNAMR241DQDEYSFQDE QDKPLPVPSN QCCNWNYFGW GEQHNDDPDS AVDDRDSDYR SETSNSIPPP301YYTTSQPNAS VHQYSVRPPP LGSRESYSDS MHSYEEFSEP QALSPTGSSR YASSGELSQG361SSQLSEDFDP DEHSLQGSDM EDERDRDSYH SCHSSVSYHK DSPRWDQDEE ELEEDLEDFL421EEEELPEDEE ELEEEEEEVP DDLGSYAQRE DVAVAEPKDF KRISLPPAAP GKEDKAPVAP481TEAPDMAKVA PKPATPDKVP AAEQIPEAEP PKDEESFRPR EDEEGQEGQD SMSRAKANWL541RAFNKVRMQL QEARGEGEMS KSLWFKGGPG GGLIIIDSMP DIRKRKPIPL VSDLAMSLVQ601SRKAGITSAL ASSTLNNEEL KNHVYKKTLQ ALIYPISCTT PHNFEVWTAT TPTYCYECEG661LLWGIARQGM RCTECGVKCH EKCQDLLNAD CLQRAAEKSS KHGAEDRTQN IIMVLKDRMK721IRERNKPEIF ELIQEIFAVT KTAHTQQMKA VKQSVLDGTS KWSAKISITV VCAQGLQAKD781KTGSSDPYVT VQVGKTKKRT KTIYGNLNPV WEENFHFECH NSSDRIKVRV WDEDDDIKSR841VKQRFKRESD DFLGQTIIEV RTLSGEMDVW YNLDKRTDKS AVSGAIRLHI SVEIKGEEKV901APYHVQYTCL HENLFHFVTD VQNNGVVKIP DAKGDDAWKV YYDETAQEIV DEFAMRYGVE961SIYQAMTHFA CLSSKYMCPG VPAVMSTLLA NINAYYAHTT ASTNVSASDR FAASNFGKER1021FVKLLDQLHN SLRIDLSMYR NNFPASSPER LQDLKSTVDL LTSITFFRMK VQELQSPPRA1081SQVVKDCVKA CLNSTYEYIF NNCHELYSRE YQTDPAKKGE VLPEEQGPSI KNLDFWSKLI1141TLIVSIIEED KNSYTPCLNQ FPQELNVGKI SAEVMWNLFA QDMKYAMEEH DKHRLCKSAD1201YMNLHFKVKW LYNEYVTELP AFKDRVPEYP AWFEPFVIQW LDENEEVSRD FLHGALERDK1261KDGFQQTSEH ALFSCSVVDV FSQLNQSFEI IKKLECPDPQ IVGHYMRRFA KTISNVLLQY1321ADIISKDFAS YCSKEKEKVP CILMNNTQQL RVQLEKMFEA MGGKELDAEA SDILKELQVK1381LNNVLDELSR VFATSFQPHI EECVKQMGDI LSQVKGTGNV PASACSSVAQ DADNVLQPIM1441DLLDSNLTLF AKICEKTVLK RVLKELWKLV MNTMEKTIVL PPLTDQTMIG NLLRKHGKGL1501EKGRVKLPSH SDGTQMIFNA AKELGQLSKL KDHMVREEAK SLTPKQCAVV ELALDTIKQY1561FHAGGVGLKK TFLEKSPDLQ SLRYALSLYT QATDLLIKTF VQTQSAQGLG VEDPVGEVSV1621HVELFTHPGT GEHKVTVKVV AANDLKWQTS GIFRPFIEVN IIGPQLSDKK RKFATKSKNN1681SWAPKYNESF QFTLSADAGP ECYELQVCVK DYCFAREDRT VGLAVLQLRE LAQRGSAACW1741LPLGRRIHMD DTGLTVLRIL SQRSNDEVAK EFVKLKSDTR SAEEGGAAPA PTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1791 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
