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UHRF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UHRF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114356-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UHRF1 (NM_013282), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HUHRF1 | HuNp95 | Nuclear protein 95 | RING finger protein 106 | UHRF1 | RNF106 | Transcription factor ICBP90 | HNP95 | ICBP90 | Np95
Origin Species
Human
NCBI GenBank
NM_013282
SwissProt
Q96T88

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGVFAVPPLS SDTMWIQVRT MDGRQTHTVD SLSRLTKVEE LRRKIQELFH VEPGLQRLFY
61RGKQMEDGHT LFDYEVRLND TIQLLVRQSL VLPHSTKERD SELSDTDSGC CLGQSESDKS
121STHGEAAAET DSRPADEDMW DETELGLYKV NEYVDARDTN MGAWFEAQVV RVTRKAPSRD
181EPCSSTSRPA LEEDVIYHVK YDDYPENGVV QMNSRDVRAR ARTIIKWQDL EVGQVVMLNY
241NPDNPKERGF WYDAEISRKR ETRTARELYA NVVLGDDSLN DCRIIFVDEV FKIERPGEGS
301PMVDNPMRRK SGPSCKHCKD DVNRLCRVCA CHLCGGRQDP DKQLMCDECD MAFHIYCLDP
361PLSSVPSEDE WYCPECRNDA SEVVLAGERL RESKKKAKMA SATSSSQRDW GKGMACVGRT
421KECTIVPSNH YGPIPGIPVG TMWRFRVQVS ESGVHRPHVA GIHGRSNDGA YSLVLAGGYE
481DDVDHGNFFT YTGSGGRDLS GNKRTAEQSC DQKLTNTNRA LALNCFAPIN DQEGAEAKDW
541RSGKPVRVVR NVKGGKNSKY APAEGNRYDG IYKVVKYWPE KGKSGFLVWR YLLRRDDDEP
601GPWTKEGKDR IKKLGLTMQY PEGYLEALAN REREKENSKR EEEEQQEGGF ASPRTGKGKW
661KRKSAGGGPS RAGSPRRTSK KTKVEPYSLT AQQSSLIRED KSNAKLWNEV LASLKDRPAS
721GSPFQLFLSK VEETFQCICC QELVFRPITT VCQHNVCKDC LDRSFRAQVF SCPACRYDLG
781RSYAMQVNQP LQTVLNQLFP GYGNGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

806 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.