Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UFD2A / UBE4B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115564-100
Catalog Number: LS-G115564
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBE4B (NM_006048), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0684 | HDNB1 | Homologous to yeast UFD2 | UFD2 | UFD2A | UBE4B | Ubiquitination factor E4B | UBOX3 | E4
- Origin Species
- Human
- NCBI GenBank
- NM_006048
- SwissProt
- O95155
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 133.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEELSADEIR RRRLARLAGG QTSQPTTPLT SPQRENPPGP PIAASAPGPS QSLGLNVHNM61TPATSPIGAS GVAHRSQSSE GVSSLSSSPS NSLETQSQSL SRSQSMDIDG VSCEKSMSQV121DVDSGIENME VDENDRREKR SLSDKEPSSG PEVSEEQALQ LVCKIFRVSW KDRDRDVIFL181SSLSAQFKQN PKEVFSDFKD LIGQILMEVL MMSTQTRDEN PFASLTATSQ PIAAAARSPD241RNLLLNTGSN PGTSPMFCSV ASFGASSLSS LGASGGASNW DSYSDHFTIE TCKETDMLNY301LIECFDRVGI EEKKAPKMCS QPAVSQLLSN IRSQCISHTA LVLQGSLTQP RSLQQPSFLV361PYMLCRNLPY GFIQELVRTT HQDEEVFKQI FIPILQGLAL AAKECSLDSD YFKYPLMALG421ELCETKFGKT HPVCNLVASL RLWLPKSLSP GCGRELQRLS YLGAFFSFSV FAEDDVKVVE481KYFSGPAITL ENTRVVSQSL QHYLELGRQE LFKILHSILL NGETREAALS YMAAVVNANM541KKAQMQTDDR LVSTDGFMLN FLWVLQQLST KIKLETVDPT YIFHPRCRIT LPNDETRVNA601TMEDVNDWLT ELYGDQPPFS EPKFPTECFF LTLHAHHLSI LPSCRRYIRR LRAIRELNRT661VEDLKNNESQ WKDSPLATRH REMLKRCKTQ LKKLVRCKAC ADAGLLDESF LRRCLNFYGL721LIQLLLRILD PAYPDITLPL NSDVPKVFAA LPEFYVEDVA EFLFFIVQYS PQALYEPCTQ781DIVMFLVVML CNQNYIRNPY LVAKLVEVMF MTNPAVQPRT QKFFEMIENH PLSTKLLVPS841LMKFYTDVEH TGATSEFYDK FTIRYHISTI FKSLWQNIAH HGTFMEEFNS GKQFVRYINM901LINDTTFLLD ESLESLKRIH EVQEEMKNKE QWDQLPRDQQ QARQSQLAQD ERVSRSYLAL961ATETVDMFHI LTKQVQKPFL RPELGPRLAA MLNFNLQQLC GPKCRDLKVE NPEKYGFEPK1021KLLDQLTDIY LQLDCARFAK AIADDQRSYS KELFEEVISK MRKAGIKSTI AIEKFKLLAE1081KVEEIVAKNA RAEIDYSDAP DEFRDPLMDT LMTDPVRLPS GTIMDRSIIL RHLLNSPTDP1141FNRQTLTESM LEPVPELKEQ IQAWMREKQN SDHSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1173 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.