LSBio
Human UFD2A / UBE4B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human UFD2A / UBE4B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all UBE4B antibodies →
$153.00each
Catalog Number: LS-G89456-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human UBE4B (NM_006048) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0684 | HDNB1 | Homologous to yeast UFD2 | UFD2 | UFD2A | UBE4B | Ubiquitination factor E4B | UBOX3 | E4
Origin Species
Human
NCBI GenBank
NM_006048
SwissProt
O95155

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEELSADEIR RRRLARLAGG QTSQPTTPLT SPQRENPPGP PIAASAPGPS QSLGLNVHNM
61TPATSPIGAS GVAHRSQSSE GVSSLSSSPS NSLETQSQSL SRSQSMDIDG VSCEKSMSQV
121DVDSGIENME VDENDRREKR SLSDKEPSSG PEVSEEQALQ LVCKIFRVSW KDRDRDVIFL
181SSLSAQFKQN PKEVFSDFKD LIGQILMEVL MMSTQTRDEN PFASLTATSQ PIAAAARSPD
241RNLLLNTGSN PGTSPMFCSV ASFGASSLSS LGASGGASNW DSYSDHFTIE TCKETDMLNY
301LIECFDRVGI EEKKAPKMCS QPAVSQLLSN IRSQCISHTA LVLQGSLTQP RSLQQPSFLV
361PYMLCRNLPY GFIQELVRTT HQDEEVFKQI FIPILQGLAL AAKECSLDSD YFKYPLMALG
421ELCETKFGKT HPVCNLVASL RLWLPKSLSP GCGRELQRLS YLGAFFSFSV FAEDDVKVVE
481KYFSGPAITL ENTRVVSQSL QHYLELGRQE LFKILHSILL NGETREAALS YMAAVVNANM
541KKAQMQTDDR LVSTDGFMLN FLWVLQQLST KIKLETVDPT YIFHPRCRIT LPNDETRVNA
601TMEDVNDWLT ELYGDQPPFS EPKFPTECFF LTLHAHHLSI LPSCRRYIRR LRAIRELNRT
661VEDLKNNESQ WKDSPLATRH REMLKRCKTQ LKKLVRCKAC ADAGLLDESF LRRCLNFYGL
721LIQLLLRILD PAYPDITLPL NSDVPKVFAA LPEFYVEDVA EFLFFIVQYS PQALYEPCTQ
781DIVMFLVVML CNQNYIRNPY LVAKLVEVMF MTNPAVQPRT QKFFEMIENH PLSTKLLVPS
841LMKFYTDVEH TGATSEFYDK FTIRYHISTI FKSLWQNIAH HGTFMEEFNS GKQFVRYINM
901LINDTTFLLD ESLESLKRIH EVQEEMKNKE QWDQLPRDQQ QARQSQLAQD ERVSRSYLAL
961ATETVDMFHI LTKQVQKPFL RPELGPRLAA MLNFNLQQLC GPKCRDLKVE NPEKYGFEPK
1021KLLDQLTDIY LQLDCARFAK AIADDQRSYS KELFEEVISK MRKAGIKSTI AIEKFKLLAE
1081KVEEIVAKNA RAEIDYSDAP DEFRDPLMDT LMTDPVRLPS GTIMDRSIIL RHLLNSPTDP
1141FNRQTLTESM LEPVPELKEQ IQAWMREKQN SDH
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1173 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.