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UCKL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UCKL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UCKL1 antibodies →
$504.00each
Catalog Number: LS-G131019-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UCKL1 (NM_001193379), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Uridine kinase-like 1 | UCKL1 | URKL1 | UCK1-LIKE | UCK1L | Uridine-cytidine kinase-like 1
Origin Species
Human
NCBI GenBank
NM_017859
SwissProt
Q9NWZ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSPPAYPGI RISGCRALGA EGSSNAESLD RLLPPVGTGR SPRKRTTSQC KSEPPLLRTS
61KRTIYTAGRP PWYNEHGTQS KEAFAIGLGG GSASGKTTVA RMIIEALDVP WVVLLSMDSF
121YKVLTEQQQE QAAHNNFNFD HPDAFDFDLI ISTLKKLKQG KSVKVPIYDF TTHSRKKDWK
181TLYGANVIIF EGIMAFADKT LLELLDMKIF VDTDSDIRLV RRLRRDISER GRDIEGVIKQ
241YNKFVKPSFD QYIQPTMRLA DIVVPRGSGN TVAIDLIVQH VHSQLEEREL SVRAALASAH
301QCHPLPRTLS VLKSTPQVRG MHTIIRDKET SRDEFIFYSK RLMRLLIEHA LSFLPFQDCV
361VQTPQGQDYA GKCYAGKQIT GVSILRAGET MEPALRAVCK DVRIGTILIQ TNQLTGEPEL
421HYLRLPKDIS DDHVILMDCT VSTGAAAMMA VRVLLDHDVP EDKIFLLSLL MAEMGVHSVA
481YAFPRVRIIT TAVDKRVNDL FRIIPGIGNF GDRYFGTDAV PDGSDEEEVA YTG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

533 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.