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UCKL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UCKL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UCKL1 antibodies →
$725.00each
Catalog Number: LS-G111994-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UCKL1 (NM_017859), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Uridine kinase-like 1 | UCKL1 | URKL1 | UCK1-LIKE | UCK1L | Uridine-cytidine kinase-like 1
Origin Species
Human
NCBI GenBank
NM_017859
SwissProt
Q9NWZ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPPARADA DPSPTSPPTA RDTPGRQAEK SETACEDRNA ESLDRLLPPV GTGRSPRKRT
61TSQCKSEPPL LRTSKRTIYT AGRPPWYNEH GTQSKEAFAI GLGGGSASGK TTVARMIIEA
121LDVPWVVLLS MDSFYKVLTE QQQEQAAHNN FNFDHPDAFD FDLIISTLKK LKQGKSVKVP
181IYDFTTHSRK KDWKTLYGAN VIIFEGIMAF ADKTLLELLD MKIFVDTDSD IRLVRRLRRD
241ISERGRDIEG VIKQYNKFVK PSFDQYIQPT MRLADIVVPR GSGNTVAIDL IVQHVHSQLE
301ERELSVRAAL ASAHQCHPLP RTLSVLKSTP QVRGMHTIIR DKETSRDEFI FYSKRLMRLL
361IEHALSFLPF QDCVVQTPQG QDYAGKCYAG KQITGVSILR AGETMEPALR AVCKDVRIGT
421ILIQTNQLTG EPELHYLRLP KDISDDHVIL MDCTVSTGAA AMMAVRVLLD HDVPEDKIFL
481LSLLMAEMGV HSVAYAFPRV RIITTAVDKR VNDLFRIIPG IGNFGDRYFG TDAVPDGSDE
541EEVAYTG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

547 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.