Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UBTF / UBF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119933-100
Catalog Number: LS-G119933
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBF1(UBTF) (NM_001076684), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Autoantigen NOR-90 | UBF | UBTF | NOR-90 | UBF-1 | UBF1 | Upstream-binding factor 1
- Origin Species
- Human
- NCBI GenBank
- NM_014233
- SwissProt
- P17480
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNGEADCPTD LEMAAPKGQD RWSQEDMLTL LECMKNNLPS NDSSKFKTTE SHMDWEKVAF61KDFSGDMCKL KWVEISNEVR KFRTLTELIL DAQEHVKNPY KGKKLKKHPD FPKKPLTPYF121RFFMEKRAKY AKLHPEMSNL DLTKILSKKY KELPEKKKMK YIQDFQREKQ EFERNLARFR181EDHPDLIQNA KKSDIPEKPK TPQQLWYTHE KKVYLKVRPD EIMRDYIQKH PELNISEEGI241TKSTLTKAER QLKDKFDGRP TKPPPNSYSL YCAELMANMK DVPSTERMVL CSQQWKLLSQ301KEKDAYHKKC DQKKKDYEVE LLRFLESLPE EEQQRVLGEE KMLNINKKQA TSPASKKPAQ361EGGKGGSEKP KRPVSAMFIF SEEKRRQLQE ERPELSESEL TRLLARMWND LSEKKKAKYK421AREAALKAQS ERKPGGEREE RGKLPESPKR AEEIWQQSVI GDYLARFKND RVKALKAMEM481TWNNMEKKEK LMWIKKAAED QKRYERELSE MRAPPAATNS SKKMKFQGEP KKPPMNGYQK541FSQELLSNGE LNHLPLKERM VEIGSRWQRI SQSQKEHYKK LAEEQQKQYK VHLDLWVKSL601SPQDRAAYKE YISNKRKSMT KLRGPNPKSS RTTLQSKSES EEDDEEDEDD EDEDEEEEDD661ENGDSSEDGG DSSESSSEDE SEDGDENEED DEDEDDDEDD DEDEDNESEG SSSSSSSSGD721SSDSDSNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
727 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.