UBTF / UBF Antibody
Catalog Number: LS-C822614-100
100 µg / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human UBTF / UBF Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G77771-20
Catalog Number: LS-G77771
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human UBF1(UBTF) (NM_001076683) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Autoantigen NOR-90 | UBF | UBTF | NOR-90 | UBF-1 | UBF1 | Upstream-binding factor 1
- Origin Species
- Human
- NCBI GenBank
- NM_014233
- SwissProt
- P17480
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNGEADCPTD LEMAAPKGQD RWSQEDMLTL LECMKNNLPS NDSSKFKTTE SHMDWEKVAF61KDFSGDMCKL KWVEISNEVR KFRTLTELIL DAQEHVKNPY KGKKLKKHPD FPKKPLTPYF121RFFMEKRAKY AKLHPEMSNL DLTKILSKKY KELPEKKKMK YIQDFQREKQ EFERNLARFR181EDHPDLIQNA KKSDIPEKPK TPQQLWYTHE KKVYLKVRPD EIMRDYIQKH PELNISEEGI241TKSTLTKAER QLKDKFDGRP TKPPPNSYSL YCAELMANMK DVPSTERMVL CSQQWKLLSQ301KEKDAYHKKC DQKKKDYEVE LLRFLESLPE EEQQRVLGEE KMLNINKKQA TSPASKKPAQ361EGGKGGSEKP KRPVSAMFIF SEEKRRQLQE ERPELSESEL TRLLARMWND LSEKKKAKYK421AREAALKAQS ERKPGGEREE RGKLPESPKR AEEIWQQSVI GDYLARFKND RVKALKAMEM481TWNNMEKKEK LMWIKKAAED QKRYERELSE MRAPPAATNS SKKMKFQGEP KKPPMNGYQK541FSQELLSNGE LNHLPLKERM VEIGSRWQRI SQSQKEHYKK LAEEQQKQYK VHLDLWVKSL601SPQDRAAYKE YISNKRKSMT KLRGPNPKSS RTTLQSKSES EEDDEEDEDD EDEDEEEEDD661ENGDSSEDGG DSSESSSEDE SEDGDENEED DEDEDDDEDD DEDEDNESEG SSSSSSSSGD721SSDSDSNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
727 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
