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UBOX5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UBOX5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113582-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBOX5 (NM_014948), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0860 | RNF37 | U-box domain containing 5 | UIP5 | RING finger protein 37 | UBCE7IP5 | UBOX5
Origin Species
Human
NCBI GenBank
NM_014948
SwissProt
O94941

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVINLCLPQF RPRIHCNKIS ADGYEVENLI SEDLTKRSHG FRTEYFIKPP VYVTVSFPFN
61VEICRINIDL TAGGGQNVTG LEMYTSASSS RVSWNTPQCR TLGPAEPSVP DKEAFTLVGK
121VLLKNQSQVV FSHRGFKARP PFGAMEATLP SPAVVAQELW NKGALSLSHV AHLRICITHV
181TGGGIPCIKR LEVWGQPAKT CSQEVIDSIL LVTSENLPQD VALQAPALPM ESDCDPGDQP
241ESQQAPSSLQ KLAEIIQDVP EEFLDPITLE IMPCPMLLPS GKVIDQSTLE KCNRSEATWG
301RVPSDPFTGV AFTPHSQPLP HPSLKARIDH FLLQHSIPGC HLLGRAQTAL AVIPSSIVLP
361SQKRKIEQAE HVPDSNFGVN ASCFSATSPL VLPTTSEHTA KKMKATNEPS LTHMDCSTGP
421LSHEQKLSQS LEIALASTLG SMPSFTARLT RGQLQHLGTR GSNTSWRPGT GSEQPGSILG
481PECASCKRVF SPYFKKEPVY QLPCGHLLCR PCLGEKQRSL PMTCTACQRP VASQDVLRVH
541FTRTRPLEQK LISEEDLAAN DILDYKDDDD KV

572 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.