Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UBA3 / UBE1C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103740-100
Catalog Number: LS-G103740
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBE1C(UBA3) (NM_198195), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HUBA3 | UBE1C | Ubiquitin-activating enzyme 3 | NEDD8-activating enzyme E1C | Nedd8-activating enzyme hUba3 | UBA3
- Origin Species
- Human
- NCBI GenBank
- NM_003968
- SwissProt
- Q8TBC4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADGEEPMAV DGGCGDTGDW EGRWNHVKKF LERSGPFTHP DFEPSTESLQ FLLDTCKVLV61IGAGGLGCEL LKNLALSGFR QIHVIDMDTI DVSNLNRQFL FRPKDIGRPK AEVAAEFLND121RVPNCNVVPH FNKIQDFNDT FYRQFHIIVC GLDSIIARRW INGMLISLLN YEDGVLDPSS181IVPLIDGGTE GFKGNARVIL PGMTACIECT LELYPPQVNF PMCTIASMPR LPEHCIEYVR241MLQWPKEQPF GEGVPLDGDD PEHIQWIFQK SLERASQYNI RGVTYRLTQG VVKRIIPAVA301STNAVIAAVC ATEVFKIATS AYIPLNNYLV FNDVDGLYTY TFEAERKENC PACSQLPQNI361QFSPSAKLQE VLDYLTNSAS LQMKSPAITA TLEGKNRTLY LQSVTSIEER TRPNLSKTLK421ELGLVDGQEL AVADVTTPQT VLFKLHFTSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
449 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.