UBA3 / UBE1C Antibody
Catalog Number: LS-C676667
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human UBA3 / UBE1C Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G82235-20
Catalog Number: LS-G82235
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human UBE1C(UBA3) (NM_198195) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HUBA3 | UBE1C | Ubiquitin-activating enzyme 3 | NEDD8-activating enzyme E1C | Nedd8-activating enzyme hUba3 | UBA3
- Origin Species
- Human
- NCBI GenBank
- NM_003968
- SwissProt
- Q8TBC4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADGEEPMAV DGGCGDTGDW EGRWNHVKKF LERSGPFTHP DFEPSTESLQ FLLDTCKVLV61IGAGGLGCEL LKNLALSGFR QIHVIDMDTI DVSNLNRQFL FRPKDIGRPK AEVAAEFLND121RVPNCNVVPH FNKIQDFNDT FYRQFHIIVC GLDSIIARRW INGMLISLLN YEDGVLDPSS181IVPLIDGGTE GFKGNARVIL PGMTACIECT LELYPPQVNF PMCTIASMPR LPEHCIEYVR241MLQWPKEQPF GEGVPLDGDD PEHIQWIFQK SLERASQYNI RGVTYRLTQG VVKRIIPAVA301STNAVIAAVC ATEVFKIATS AYIPLNNYLV FNDVDGLYTY TFEAERKENC PACSQLPQNI361QFSPSAKLQE VLDYLTNSAS LQMKSPAITA TLEGKNRTLY LQSVTSIEER TRPNLSKTLK421ELGLVDGQEL AVADVTTPQT VLFKLHFTSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
449 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
