Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Tuberin / TSC2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124356-100
Catalog Number: LS-G124356
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Tuberin(TSC2) (NM_001114382), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LAM | Tuberous sclerosis 2 protein | TSC4 | Tuberin | Tuberous sclerosis 2 | TSC2
- Origin Species
- Human
- NCBI GenBank
- NM_000548
- SwissProt
- P49815
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 197.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKPTSKDSG LKEKFKILLG LGTPRPNPRS AEGKQTEFII TAEILRELSM ECGLNNRIRM61IGQICEVAKT KKFEEHAVEA LWKAVADLLQ PERPLEARHA VLALLKAIVQ GQGERLGVLR121ALFFKVIKDY PSNEDLHERL EVFKALTDNG RHITYLEEEL ADFVLQWMDV GLSSEFLLVL181VNLVKFNSCY LDEYIARMVQ MICLLCVRTA SSVDIEVSLQ VLDAVVCYNC LPAESLPLFI241VTLCRTINVK ELCEPCWKLM RNLLGTHLGH SAIYNMCHLM EDRAYMEDAP LLRGAVFFVG301MALWGAHRLY SLRNSPTSVL PSFYQAMACP NEVVSYEIVL SITRLIKKYR KELQVVAWDI361LLNIIERLLQ QLQTLDSPEL RTIVHDLLTT VEELCDQNEF HGSQERYFEL VERCADQRPE421SSLLNLISYR AQSIHPAKDG WIQNLQALME RFFRSESRGA VRIKVLDVLS FVLLINRQFY481EEELINSVVI SQLSHIPEDK DHQVRKLATQ LLVDLAEGCH THHFNSLLDI IEKVMARSLS541PPPELEERDV AAYSASLEDV KTAVLGLLVI LQTKLYTLPA SHATRVYEML VSHIQLHYKH601SYTLPIASSI RLQAFDFLLL LRADSLHRLG LPNKDGVVRF SPYCVCDYME PERGSEKKTS661GPLSPPTGPP GPAPAGPAVR LGSVPYSLLF RVLLQCLKQE SDWKVLKLVL GRLPESLRYK721VLIFTSPCSV DQLCSALCSM LSGPKTLERL RGAPEGFSRT DLHLAVVPVL TALISYHNYL781DKTKQREMVY CLEQGLIHRC ASQCVVALSI CSVEMPDIII KALPVLVVKL THISATASMA841VPLLEFLSTL ARLPHLYRNF AAEQYASVFA ISLPYTNPSK FNQYIVCLAH HVIAMWFIRC901RLPFRKDFVP FITKGLRSNV LLSFDDTPEK DSFRARSTSL NERPKSLRIA RPPKQGLNNS961PPVKEFKESS AAEAFRCRSI SVSEHVVRSR IQTSLTSASL GSADENSVAQ ADDSLKNLHL1021ELTETCLDMM ARYVFSNFTA VPKRSPVGEF LLAGGRTKTW LVGNKLVTVT TSVGTGTRSL1081LGLDSGELQS GPESSSSPGV HVRQTKEAPA KLESQAGQQV SRGARDRVRS MSGGHGLRVG1141ALDVPASQFL GSATSPGPRT APAAKPEKAS AGTRVPVQEK TNLAAYVPLL TQGWAEILVR1201RPTGNTSWLM SLENPLSPFS SDINNMPLQE LSNALMAAER FKEHRDTALY KSLSVPAAST1261AKPPPLPRSN TDSAVVMEEG SPGEVPVLVE PPGLEDVEAA LGMDRRTDAY SRSSSVSSQE1321EKSLHAEELV GRGIPIERVV SSEGGRPSVD LSFQPSQPLS KSSSSPELQT LQDILGDPGD1381KADVGRLSPE VKARSQSGTL DGESAAWSAS GEDSRGQPEG PLPSSSPRSP SGLRPRGYTI1441SDSAPSRRGK RVERDALKSR ATASNAEKVP GINPSFVFLQ LYHSPFFGDE SNKPILLPNE1501SQSFERSVQL LDQIPSYDTH KIAVLYVGEG QSNSELAILS NEHGSYRYTE FLTGLGRLIE1561LKDCQPDKVY LGGLDVCGED GQFTYCWHDD IMQAVFHIAT LMPTKDVDKH RCDKKRHLGN1621DFVSIVYNDS GEDFKLGTIK GQFNFVHVIV TPLDYECNLV SLQCRKDMEG LVDTSVAKIV1681SDRNLPFVAR QMALHANMAS QVHHSRSNPT DIYPSKWIAR LRHIKRLRQR ICEEAAYSNP1741SLPLVHPPSH SKAPAQTPAE PTPGYEVGQR KRLISSVEDF TEFVLEQKLISEEDLAANDILDYKDDDDKV
1784 aa + 26 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.