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TTLL7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTLL7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109836-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTLL7 (NM_024686), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TTLL7 | RP5-836J3.2 | Tubulin polyglutamylase TTLL7
Origin Species
Human
NCBI GenBank
NM_024686
SwissProt
Q6ZT98

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSLPQEGVI QGPSPLDLNT ELPYQSTMKR KVRKKKKKGT ITANVAGTKF EIVRLVIDEM
61GFMKTPDEDE TSNLIWCDSA VQQEKISELQ NYQRINHFPG MGEICRKDFL ARNMTKMIKS
121RPLDYTFVPR TWIFPAEYTQ FQNYVKELKK KRKQKTFIVK PANGAMGHGI SLIRNGDKLP
181SQDHLIVQEY IEKPFLMEGY KFDLRIYILV TSCDPLKIFL YHDGLVRMGT EKYIPPNESN
241LTQLYMHLTN YSVNKHNEHF ERDETENKGS KRSIKWFTEF LQANQHDVAK FWSDISELVV
301KTLIVAEPHV LHAYRMCRPG QPPGSESVCF EVLGFDILLD RKLKPWLLEI NRAPSFGTDQ
361KIDYDVKRGV LLNALKLLNI RTSDKRRNLA KQKAEAQRRL YGQNSIKRLL PGSSDWEQQR
421HQLERRKEEL KERLAQVRKQ ISREEHENRH MGNYRRIYPP EDKALLEKYE NLLAVAFQTF
481LSGRAASFQR ELNNPLKRMK EEDILDLLEQ CEIDDEKLMG KTTKTRGPKP LCSMPESTEI
541MKRPKYCSSD SSYDSSSSSS ESDENEKEEY QNKKREKQVT YNLKPSNHYK LIQQPSSIRR
601SVSCPRSISA QSPSSGDTRP FSAQQMISVS RPTSASRSHS LNRASSYMRH LPHSNDACST
661NSQVSESLRQ LKTKEQEDDL TSQTLFVLKD MKIRFPGKSD AESELLIEDI IDNWKYHKTK
721VASYWLIKLD SVKQRKVLDI VKTSIRTVLP RIWKVPDVEE VNLYRIFNRV FNRLLWSRGQ
781GLWNCFCDSG SSWESIFNKS PEVVTPLQLQ CCQRLVELCK QCLLVVYKYA TDKRGSLSGI
841GPDWGNSRYL LPGSTQFFLR TPTYNLKYNS PGMTRSNVLF TSRYGHL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

887 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.