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Human TTLL7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TTLL7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all TTLL7 antibodies →
$220.00each
Catalog Number: LS-G86114-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TTLL7 (NM_024686) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TTLL7 | RP5-836J3.2 | Tubulin polyglutamylase TTLL7
Origin Species
Human
NCBI GenBank
NM_024686
SwissProt
Q6ZT98

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSLPQEGVI QGPSPLDLNT ELPYQSTMKR KVRKKKKKGT ITANVAGTKF EIVRLVIDEM
61GFMKTPDEDE TSNLIWCDSA VQQEKISELQ NYQRINHFPG MGEICRKDFL ARNMTKMIKS
121RPLDYTFVPR TWIFPAEYTQ FQNYVKELKK KRKQKTFIVK PANGAMGHGI SLIRNGDKLP
181SQDHLIVQEY IEKPFLMEGY KFDLRIYILV TSCDPLKIFL YHDGLVRMGT EKYIPPNESN
241LTQLYMHLTN YSVNKHNEHF ERDETENKGS KRSIKWFTEF LQANQHDVAK FWSDISELVV
301KTLIVAEPHV LHAYRMCRPG QPPGSESVCF EVLGFDILLD RKLKPWLLEI NRAPSFGTDQ
361KIDYDVKRGV LLNALKLLNI RTSDKRRNLA KQKAEAQRRL YGQNSIKRLL PGSSDWEQQR
421HQLERRKEEL KERLAQVRKQ ISREEHENRH MGNYRRIYPP EDKALLEKYE NLLAVAFQTF
481LSGRAASFQR ELNNPLKRMK EEDILDLLEQ CEIDDEKLMG KTTKTRGPKP LCSMPESTEI
541MKRPKYCSSD SSYDSSSSSS ESDENEKEEY QNKKREKQVT YNLKPSNHYK LIQQPSSIRR
601SVSCPRSISA QSPSSGDTRP FSAQQMISVS RPTSASRSHS LNRASSYMRH LPHSNDACST
661NSQVSESLRQ LKTKEQEDDL TSQTLFVLKD MKIRFPGKSD AESELLIEDI IDNWKYHKTK
721VASYWLIKLD SVKQRKVLDI VKTSIRTVLP RIWKVPDVEE VNLYRIFNRV FNRLLWSRGQ
781GLWNCFCDSG SSWESIFNKS PEVVTPLQLQ CCQRLVELCK QCLLVVYKYA TDKRGSLSGI
841GPDWGNSRYL LPGSTQFFLR TPTYNLKYNS PGMTRSNVLF TSRYGHL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

887 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.