Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TTF1 / Txn Termination Factor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114704-100
Catalog Number: LS-G114704
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Transcription termination factor 1(TTF1) (NM_007344), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TTF-I | TTF1 | TTF-1
- Origin Species
- Human
- NCBI GenBank
- NM_007344
- SwissProt
- Q15361
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 102.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGESSRFEI HTPVSDKKKK KCSIHKERPQ KHSHEIFRDS SLVNEQSQIT RRKKRKKDFQ61HLISSPLKKS RICDETANAT STLKKRKKRR YSALEVDEEA GVTVVLVDKE NINNTPKHFR121KDVDVVCVDM SIEQKLPRKP KTDKFQVLAK SHAHKSEALH SKVREKKNKK HQRKAASWES181QRARDTLPQS ESHQEESWLS VGPGGEITEL PASAHKNKSK KKKKKSSNRE YETLAMPEGS241QAGREAGTDM QESQPTVGLD DETPQLLGPT HKKKSKKKKK KKSNHQEFEA LAMPEGSQVG301SEVGADMQES RPAVGLHGET AGIPAPAYKN KSKKKKKKSN HQEFEAVAMP ESLESAYPEG361SQVGSEVGTV EGSTALKGFK ESNSTKKKSK KRKLTSVKRA RVSGDDFSVP SKNSESTLFD421SVEGDGAMME EGVKSRPRQK KTQACLASKH VQEAPRLEPA NEEHNVETAE DSEIRYLSAD481SGDADDSDAD LGSAVKQLQE FIPNIKDRAT STIKRMYRDD LERFKEFKAQ GVAIKFGKFS541VKENKQLEKN VEDFLALTGI ESADKLLYTD RYPEEKSVIT NLKRRYSFRL HIGRNIARPW601KLIYYRAKKM FDVNNYKGRY SEGDTEKLKM YHSLLGNDWK TIGEMVARSS LSVALKFSQI661SSQRNRGAWS KSETRKLIKA VEEVILKKMS PQELKEVDSK LQENPESCLS IVREKLYKGI721SWVEVEAKVQ TRNWMQCKSK WTEILTKRMT NGRRIYYGMN ALRAKVSLIE RLYEINVEDT781NEIDWEDLAS AIGDVPPSYV QTKFSRLKAV YVPFWQKKTF PEIIDYLYET TLPLLKEKLE841KMMEKKGTKI QTPAAPKQVF PFRDIFYYED DSEGEDIEKE SEGQAPCMAH ACNSSTLGGQ901GRWIITRTRPLEQKLISEEDLAANDILDYKDDDDKV
905 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.