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Human TTF1 / Txn Termination Factor Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TTF1 / Txn Termination Factor Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G83643-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Transcription termination factor 1(TTF1) (NM_007344) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TTF-I | TTF1 | TTF-1
Origin Species
Human
NCBI GenBank
NM_007344
SwissProt
Q15361

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGESSRFEI HTPVSDKKKK KCSIHKERPQ KHSHEIFRDS SLVNEQSQIT RRKKRKKDFQ
61HLISSPLKKS RICDETANAT STLKKRKKRR YSALEVDEEA GVTVVLVDKE NINNTPKHFR
121KDVDVVCVDM SIEQKLPRKP KTDKFQVLAK SHAHKSEALH SKVREKKNKK HQRKAASWES
181QRARDTLPQS ESHQEESWLS VGPGGEITEL PASAHKNKSK KKKKKSSNRE YETLAMPEGS
241QAGREAGTDM QESQPTVGLD DETPQLLGPT HKKKSKKKKK KKSNHQEFEA LAMPEGSQVG
301SEVGADMQES RPAVGLHGET AGIPAPAYKN KSKKKKKKSN HQEFEAVAMP ESLESAYPEG
361SQVGSEVGTV EGSTALKGFK ESNSTKKKSK KRKLTSVKRA RVSGDDFSVP SKNSESTLFD
421SVEGDGAMME EGVKSRPRQK KTQACLASKH VQEAPRLEPA NEEHNVETAE DSEIRYLSAD
481SGDADDSDAD LGSAVKQLQE FIPNIKDRAT STIKRMYRDD LERFKEFKAQ GVAIKFGKFS
541VKENKQLEKN VEDFLALTGI ESADKLLYTD RYPEEKSVIT NLKRRYSFRL HIGRNIARPW
601KLIYYRAKKM FDVNNYKGRY SEGDTEKLKM YHSLLGNDWK TIGEMVARSS LSVALKFSQI
661SSQRNRGAWS KSETRKLIKA VEEVILKKMS PQELKEVDSK LQENPESCLS IVREKLYKGI
721SWVEVEAKVQ TRNWMQCKSK WTEILTKRMT NGRRIYYGMN ALRAKVSLIE RLYEINVEDT
781NEIDWEDLAS AIGDVPPSYV QTKFSRLKAV YVPFWQKKTF PEIIDYLYET TLPLLKEKLE
841KMMEKKGTKI QTPAAPKQVF PFRDIFYYED DSEGEDIEKE SEGQAPCMAH ACNSSTLGGQ
901GRWII
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

905 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.