LSBio
TRPM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

TRPM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRPM3 antibodies →
$725.00each
Catalog Number: LS-G110863-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRPM3 (NM_020952), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GON-2 | KIAA1616 | LTrpC-3 | LTRPC3 | MLSN2 | TRPM3 | Melastatin 2 | Melastatin-2
Origin Species
Human
NCBI GenBank
NM_024971
SwissProt
Q9HCF6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
177.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYVRVSFDTK PDLLLHLMTK EWQLELPKLL ISVHGGLQNF ELQPKLKQVF GKGLIKAAMT
61TGAWIFTGGV NTGVIRHVGD ALKDHASKSR GKICTIGIAP WGIVENQEDL IGRDVVRPYQ
121TMSNPMSKLT VLNSMHSHFI LADNGTTGKY GAEVKLRRQL EKHISLQKIN TRIGQGVPVV
181ALIVEGGPNV ISIVLEYLRD TPPVPVVVCD GSGRASDILA FGHKYSEEGG LINESLRDQL
241LVTIQKTFTY TRTQAQHLFI ILMECMKKKE LITVFRMGSE GHQDIDLAIL TALLKGANAS
301APDQLSLALA WNRVDIARSQ IFIYGQQWPV GSLEQAMLDA LVLDRVDFVK LLIENGVSMH
361RFLTISRLEE LYNTRHGPSN TLYHLVRDVK KGNLPPDYRI SLIDIGLVIE YLMGGAYRCN
421YTRKRFRTLY HNLFGPKRPK ALKLLGMEDD IPLRRGRKTT KKREEEVDID LDDPEINHFP
481FPFHELMVWA VLMKRQKMAL FFWQHGEEAM AKALVACKLC KAMAHEASEN DMVDDISQEL
541NHNSRDFGQL AVELLDQSYK QDEQLAMKLL TYELKNWSNA TCLQLAVAAK HRDFIAHTCS
601QMLLTDMWMG RLRMRKNSGL KVILGILLPP SILSLEFKNK DDMPYMSQAQ EIHLQEKEAE
661EPEKPTKEKE EEDMELTAML GRNNGESSRK KDEEEVQSKH RLIPLGRKIY EFYNAPIVKF
721WFYTLAYIGY LMLFNYIVLV KMERWPSTQE WIVISYIFTL GIEKMREILM SEPGKLLQKV
781KVWLQEYWNV TDLIAILLFS VGMILRLQDQ PFRSDGRVIY CVNIIYWYIR LLDIFGVNKY
841LGPYVMMIGK MMIDMMYFVI IMLVVLMSFG VARQAILFPN EEPSWKLAKN IFYMPYWMIY
901GEVFADQIDP PCGQNETRED GKIIQLPPCK TGAWIVPAIM ACYLLVANIL LVNLLIAVFN
961NTFFEVKSIS NQVWKFQRYQ LIMTFHERPV LPPPLIIFSH MTMIFQHLCC RWRKHESDPD
1021ERDYGLKLFI TDDELKKVHD FEEQCIEEYF REKDDRFNSS NDERIRVTSE RVENMSMRLE
1081EVNEREHSMK ASLQTVDIRL AQLEDLIGRM ATALERLTGL ERAESNKIRS RTSSDCTDAA
1141YIVRQSSFNS QEGNTFKLQE SIDPAGEETM SPTSPTLMPR MRSHSFYSVN MKDKGGIEKL
1201ESIFKERSLS LHRATSSHSV AKEPKAPAAP ANTLAIVPDS RRPSSCIDIY VSAMDELHCD
1261IDPLDNSVNI LGLGEPSFST PVPSTAPSSS AYATLAPTDR PPSRSIDFED ITSMDTRSFS
1321SDYTHLPECQ NPWDSEPPMY HTIERSKSSR YLATTPFLLE EAPIVKSHSF MFSPSRSYYA
1381NFGVPVKTAE YTSITDCIDT RCVNAPQAIA DRAAFPGGLG DKVEDLTCCH PEREAELSHP
1441SSDSEENEAK GRRATIAISS QEGDNSERTL SNNITVPKIE RANSYSAEEP SAPYAHTRKS
1501FSISDKLDRQ RNTASLRNPF QRSKSSKPEG RGDSLSMRRL SRTSAFQSFE SKHN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1554 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.