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TRPM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRPM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRPM3 antibodies →
$725.00each
Catalog Number: LS-G109629-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRPM3 (NM_024971), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GON-2 | KIAA1616 | LTrpC-3 | LTRPC3 | MLSN2 | TRPM3 | Melastatin 2 | Melastatin-2
Origin Species
Human
NCBI GenBank
NM_024971
SwissProt
Q9HCF6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
178.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYVRVSFDTK PDLLLHLMTK EWQLELPKLL ISVHGGLQNF ELQPKLKQVF GKGLIKAAMT
61TGAWIFTGGV NTGVIRHVGD ALKDHASKSR GKICTIGIAP WGIVENQEDL IGRDVVRPYQ
121TMSNPMSKLT VLNSMHSHFI LADNGTTGKY GAEVKLRRQL EKHISLQKIN TRIGQGVPVV
181ALIVEGGPNV ISIVLEYLRD TPPVPVVVCD GSGRASDILA FGHKYSEEGG LINESLRDQL
241LVTIQKTFTY TRTQAQHLFI ILMECMKKKE LITVFRMGSE GHQDIDLAIL TALLKGANAS
301APDQLSLALA WNRVDIARSQ IFIYGQQWPV GSLEQAMLDA LVLDRVDFVK LLIENGVSMH
361RFLTISRLEE LYNTRHGPSN TLYHLVRDVK KREYPGFGWI YFKGNLPPDY RISLIDIGLV
421IEYLMGGAYR CNYTRKRFRT LYHNLFGPKR PKALKLLGME DDIPLRRGRK TTKKREEEVD
481IDLDDPEINH FPFPFHELMV WAVLMKRQKM ALFFWQHGEE AMAKALVACK LCKAMAHEAS
541ENDMVDDISQ ELNHNSRDFG QLAVELLDQS YKQDEQLAMK LLTYELKNWS NATCLQLAVA
601AKHRDFIAHT CSQMLLTDMW MGRLRMRKNS GLKVILGILL PPSILSLEFK NKDDMPYMSQ
661AQEIHLQEKE AEEPEKPTKE KEEEDMELTA MLGRNNGESS RKKDEEEVQS KHRLIPLGRK
721IYEFYNAPIV KFWFYTLAYI GYLMLFNYIV LVKMERWPST QEWIVISYIF TLGIEKMREI
781LMSEPGKLLQ KVKVWLQEYW NVTDLIAILL FSVGMILRLQ DQPFRSDGRV IYCVNIIYWY
841IRLLDIFGVN KYLGPYVMMI GKMMIDMMYF VIIMLVVLMS FGVARQAILF PNEEPSWKLA
901KNIFYMPYWM IYGEVFADQI DPPCGQNETR EDGKIIQLPP CKTGAWIVPA IMACYLLVAN
961ILLVNLLIAV FNNTFFEVKS ISNQVWKFQR YQLIMTFHER PVLPPPLIIF SHMTMIFQHL
1021CCRWRKHESD PDERDYGLKL FITDDELKKV HDFEEQCIEE YFREKDDRFN SSNDERIRVT
1081SERVENMSMR LEEVNEREHS MKASLQTVDI RLAQLEDLIG RMATALERLT GLERAESNKI
1141RSRTSSDCTD AAYIVRQSSF NSQEGNTFKL QESIDPAGEE TMSPTSPTLM PRMRSHSFYX
1201VNMKDKGGIE KLESIFKERS LSLHRATSSH SVAKEPKAPA APANTLAIVP DSRRPSSCID
1261IYVSAMDELH CDIDPLDNSV NILGLGEPSF STPVPSTAPS SSAYATLAPT DRPPSRSIDF
1321EDITSMDTRS FSSDYTHLPE CQNPWDSEPP MYHTIERSKS SRYLATTPFL LEEAPIVKSH
1381SFMFSPSRSY YANFGVPVKT AEYTSITDCI DTRCVNAPQA IADRAAFPGG LGDKVEDLTC
1441CHPEREAELS HPSSDSEENE AKGRRATIAI SSQEGDNSER TLSNNITVPK IERANSYSAE
1501EPSAPYAHTR KSFSISDKLD RQRNTASLQN PFQRSKSSKP EGRGDSLSMR RLSRTSAFQS
1561FESKHN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1566 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.