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TRHDE Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRHDE Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRHDE antibodies →
$725.00each
Catalog Number: LS-G101139-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRHDE (NM_013381), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Pyroglutamyl aminopeptidase II | Thyroliberinase | TRH-specific aminopeptidase | PAP-II | PGPEP2 | TRH-DE | TRH-degrading ectoenzyme | TRHDE | Pyroglutamyl-peptidase II
Origin Species
Human
NCBI GenBank
NM_013381
SwissProt
Q9UKU6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGEDDAALRA GSRGLSDPWA DSVGVRPRTT ERHIAVHKRL VLAFAVSLVA LLAVTMLAVL
61LSLRFDECGA SATPGADGGP SGFPERGGNG SLPGSARRNH HAGGDSWQPE AGGVASPGTT
121SAQPPSEEER EPWEPWTQLR LSGHLKPLHY NLMLTAFMEN FTFSGEVNVE IACRNATRYV
181VLHASRVAVE KVQLAEDRAF GAVPVAGFFL YPQTQVLVVV LNRTLDAQRN YNLKIIYNAL
241IENELLGFFR SSYVLHGERR FLGVTQFSPT HARKAFPCFD EPIYKATFKI SIKHQATYLS
301LSNMPVETSV FEEDGWVTDH FSQTPLMSTY YLAWAICNFT YRETTTKSGV VVRLYARPDA
361IRRGSGDYAL HITKRLIEFY EDYFKVPYSL PKLDLLAVPK HPYAAMENWG LSIFVEQRIL
421LDPSVSSISY LLDVTMVIVH EICHQWFGDL VTPVWWEDVW LKEGFAHYFE FVGTDYLYPG
481WNMEKQRFLT DVLHEVMLLD GLASSHPVSQ EVLQATDIDR VFDWIAYKKG AALIRMLANF
541MGHSVFQRGL QDYLTIHKYG NAARNDLWNT LSEALKRNGK YVNIQEVMDQ WTLQMGYPVI
601TILGNTTAEN RIIITQQHFI YDISAKTKAL KLQNNSYLWQ IPLTIVVGNR SHVSSEAIIW
661VSNKSEHHRI TYLDKGSWLL GNINQTGYFR VNYDLRNWRL LIDQLIRNHE VLSVSNRAGL
721IDDAFSLARA GYLPQNIPLE IIRYLSEEKD FLPWHAASRA LYPLDKLLDR MENYNIFNEY
781ILKQVATTYI KLGWPKNNFN GSLVQASYQH EELRREVIML ACSFGNKHCH QQASTLISDW
841ISSNRNRIPL NVRDIVYCTG VSLLDEDVWE FIWMKFHSTT AVSEKKILLE ALTCSDDRNL
901LNRLLNLSLN SEVVLDQDAI DVIIHVARNP HGRDLAWKFF RDKWKILNTR YGEALFMNSK
961LISGVTEFLN TEGELKELKN FMKNYDGVAA ASFSRAVETV EANVRWKMLY QDELFQWLGK
1021ALRH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1024 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.