TRHDE Antibody
Catalog Number: LS-C497009
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human TRHDE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89835-20
Catalog Number: LS-G89835
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TRHDE (NM_013381) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Pyroglutamyl aminopeptidase II | Thyroliberinase | TRH-specific aminopeptidase | PAP-II | PGPEP2 | TRH-DE | TRH-degrading ectoenzyme | TRHDE | Pyroglutamyl-peptidase II
- Origin Species
- Human
- NCBI GenBank
- NM_013381
- SwissProt
- Q9UKU6
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 116.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGEDDAALRA GSRGLSDPWA DSVGVRPRTT ERHIAVHKRL VLAFAVSLVA LLAVTMLAVL61LSLRFDECGA SATPGADGGP SGFPERGGNG SLPGSARRNH HAGGDSWQPE AGGVASPGTT121SAQPPSEEER EPWEPWTQLR LSGHLKPLHY NLMLTAFMEN FTFSGEVNVE IACRNATRYV181VLHASRVAVE KVQLAEDRAF GAVPVAGFFL YPQTQVLVVV LNRTLDAQRN YNLKIIYNAL241IENELLGFFR SSYVLHGERR FLGVTQFSPT HARKAFPCFD EPIYKATFKI SIKHQATYLS301LSNMPVETSV FEEDGWVTDH FSQTPLMSTY YLAWAICNFT YRETTTKSGV VVRLYARPDA361IRRGSGDYAL HITKRLIEFY EDYFKVPYSL PKLDLLAVPK HPYAAMENWG LSIFVEQRIL421LDPSVSSISY LLDVTMVIVH EICHQWFGDL VTPVWWEDVW LKEGFAHYFE FVGTDYLYPG481WNMEKQRFLT DVLHEVMLLD GLASSHPVSQ EVLQATDIDR VFDWIAYKKG AALIRMLANF541MGHSVFQRGL QDYLTIHKYG NAARNDLWNT LSEALKRNGK YVNIQEVMDQ WTLQMGYPVI601TILGNTTAEN RIIITQQHFI YDISAKTKAL KLQNNSYLWQ IPLTIVVGNR SHVSSEAIIW661VSNKSEHHRI TYLDKGSWLL GNINQTGYFR VNYDLRNWRL LIDQLIRNHE VLSVSNRAGL721IDDAFSLARA GYLPQNIPLE IIRYLSEEKD FLPWHAASRA LYPLDKLLDR MENYNIFNEY781ILKQVATTYI KLGWPKNNFN GSLVQASYQH EELRREVIML ACSFGNKHCH QQASTLISDW841ISSNRNRIPL NVRDIVYCTG VSLLDEDVWE FIWMKFHSTT AVSEKKILLE ALTCSDDRNL901LNRLLNLSLN SEVVLDQDAI DVIIHVARNP HGRDLAWKFF RDKWKILNTR YGEALFMNSK961LISGVTEFLN TEGELKELKN FMKNYDGVAA ASFSRAVETV EANVRWKMLY QDELFQWLGK1021ALRHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1024 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
