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TRERF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRERF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108232-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TReP132(TRERF1) (NM_033502), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ139D8.5 | HSA277276 | Rapa-1 | Rapa-2 | TRERF1 | RP1-139D8.5 | BCAR2 | TReP-132 | Zinc finger protein rapa | RAPA | TREP132
Origin Species
Human
SwissProt
Q96PN7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDQQLYKTN HVAHGSENLF YQQPPLGVHS GLNHNYGNAV TGGGMDAPQA SPISPHFPQD
61TRDGLGLPVG SKNLGQMDTS RQGGWGSHAG PGNHVQLRGN LANSNMMWGA PAQAEPTDGY
121QYTYSQASEI RTQKLTSGVL HKLDSFTQVF ANQNLRIQVN NMAQVLHTQS AVMDGAPDSA
181LRQLLSQKPM EPPAPAIPSR YQQVPQQPHP GFTGGLSKPA LQVGQHPTQG HLYYDYQQPL
241AQVPVQGGQP LQAPQMLSQH MQQMQQHQYY PPQQQQQAGQ QRISMQEIQT QPQQIRPSQP
301QPPPQQQQPQ QLQLQQRQGS MQIPQYYQPQ PMMQHLQEQQ QQQMHLQPPS YHRDPHQYTP
361EQAHTVQLIP LGSMSQYYYQ EPQQPYSHPL YQQSHLSQHQ QREDSQLKTY SSDRQAQAML
421SSHGDLGPPD TGMGDPASSD LTRVSSTLPH RPLLSPSGIH LNNMGPQHQQ LSPSAMWPQM
481HLPDGRAQPG SPESSGQPKG AFGEQFDAKN KLTCSICLKE FKNLPALNGH MRSHGGMRAS
541PNLKQEEGEK VLPPQPQPPL PPPPPPPPPP QLPPEAESLT PMVMPVSVPV KLLPPKPSSQ
601GFTNSTVAAP SARDKPASSM SDDEMPVLEI PRKHQPSVPK AEEPLKTVQE KKKFRHRPEP
661LFIPPPPSYN PNPAASYSGA TLYQSQLRSP RVLGDHLLLD PTHELPPYTP PPMLSPVRQG
721SGLFSNVLIS GHGPGAHPQL PLTPLTPTPR VLLCRSNSID GSNVTVTPGP GEQTVDVEPR
781INIGLRFQAE IPELQDISAL AQDTHKATLV WKPWPELENH DLQQRVENLL NLCCSSALPG
841GGTNSEFALH SLFEAKGDVM VALEMLLLRK PVRLKCHPLA NYHYAGSDKW TSLERKLFNK
901ALATYSKDFI FVQKMVKSKT VAQCVEYYYT WKKIMRLGRK HRTRLAEIID DCVTSEEEEE
961LEEEEEEDPE EDRKSTKEEE SEVPKSPEPP PVPVLAPTEG PPLQALGQPS GSFICEMPNC
1021GAVFSSRQAL NGHARIHGGT NQVTKARGAI PSGKQKPGGT QSGYCSVKSS PSHSTTSGET
1081DPTTIFPCKE CGKVFFKIKS RNAHMKTHRQ QEEQQRQKAQ KAAFAAEMAA TIERTTGPVG
1141APGLLPLDQL SLIKPIKDVD ILDDDVVQQL GGVMEEAEVV DTDLLLDDQD SVLLQGDAEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1200 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.