Fluorescent image of MCF-7 cell stained with TREF1 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). TREF1 immunoreactivity is localized to Nucleus significantly.

TRERF1 Antibody (aa1136-1164)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Flow Cytometry, Immunofluorescence, Western Blot
Reactivity:	Human
Format:	Liquid

$401.00each

Catalog Number: LS-C160168-400

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: TRERF1 antibody LS-C160168 is an unconjugated rabbit polyclonal antibody to human TRERF1 (aa1136-1164). Validated for Flow, IF and WB.
Application: FC, IF, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: DJ139D8.5 | HSA277276 | Rapa-1 | Rapa-2 | TRERF1 | RP1-139D8.5 | BCAR2 | TReP-132 | Zinc finger protein rapa | RAPA | TREP132
Recommended Dilution: FC: 1:10 - 1:50
Recommended Dilution: IF/ICC: 1:10 - 1:50
Recommended Dilution: WB: 1:1000
UniProt Number: Q96PN7
NCBI GenBank: NP_277037.1
SwissProt: Q96PN7

Target

Target: Human TRERF1
Antigen Species: Human
Epitope: This TREF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1136-1164 amino acids from the C-terminal region of human TREF1.
Gene Name: TRERF1

Antibody

Host Species: Rabbit
Clonality: Polyclonal

Immunogen

Immunogen Type: Peptide

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Volume: 400 Microliter
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer: PBS