Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRDMT1 / DNMT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100284-100
Catalog Number: LS-G100284
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Dnmt2(TRDMT1) (NM_004412), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DMNT2 | DNA MTase homolog HsaIIP | DNMT2 | M.HsaIIP | MHSAIIP | RNMT1 | TRDMT1 | DNA methyltransferase-2 | PUMET
- Origin Species
- Human
- NCBI GenBank
- NM_004412
- SwissProt
- O14717
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPLRVLELY SGVGGMHHAL RESCIPAQVV AAIDVNTVAN EVYKYNFPHT QLLAKTIEGI61TLEEFDRLSF DMILMSPPCQ PFTRIGRQGD MTDSRTNSFL YILDILPRLQ KLPKYILLEN121VKGFEVSSTR DLLIQTIENC GFQYQEFLLS PTSLGIPNSR LRYFLIAKLQ SEPLPFQAPG181QVLMEFPKIE SVHPQKYAMD VENKIQEKNV EPNISFDGSI QCSGKDAILF KLETAEEIHR241KNQQDSDLSV KMLKDFLEDD TDVNQYLLPP KSLLRYALLL DIVQPTCRRS VCFTKGYGSY301IEGTGSVLQT AEDVQVENIY KSLTNLSQEE QITKLLILKL RYFTPKEIAN LLGFPPEFGF361PEKITVKQRY RLLGNSLNVH VVAKLIKILY ETRTRPLEQKLISEEDLAANDILDYKDDDDKV
391 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.