TRDMT1 / DNMT2 Antibody
Catalog Number: LS-C665163-100
100 µg / $348.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human TRDMT1 / DNMT2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G80192-20
Catalog Number: LS-G80192
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Dnmt2(TRDMT1) (NM_176083) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DMNT2 | DNA MTase homolog HsaIIP | DNMT2 | M.HsaIIP | MHSAIIP | RNMT1 | TRDMT1 | DNA methyltransferase-2 | PUMET
- Origin Species
- Human
- NCBI GenBank
- NM_004412
- SwissProt
- O14717
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPLRVLELY SGVGGMHHAL RESCIPAQVV AAIDVNTVAN EVYKYNFPHT QLLAKTIEGI61TLEEFDRLSF DMILMSPPCQ PFTRLQKLPK YILLENVKGF EVSSTRDLLI QTIENCGFQY121QEFLLSPTSL GIPNSRLRYF LIAKLQSEPL PFQAPGQVLM EFPKIESVHP QKYAMDVENK181IQEKNVEPNI SFDGSIQCSG KDAILFKLET AEEIHRKNQQ DSDLSVKMLK DFLEDDTDVN241QYLLPPKSLL RYALLLDIVQ PTCRRSVCFT KGYGSYIEGT GSVLQTAEDV QVENIYKSLT301NLSQEEQITK LLILKLRYFT PKEIANLLGF PPEFGFPEKI TVKQRYRLLG NSLNVHVVAK361LIKILYETRTRPLEQKLISEEDLAANDILDYKDDDDKV
367 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
