Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRB3 / TRIB3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101732-100
Catalog Number: LS-G101732
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIB3 (NM_021158), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C20orf97 | NIPK | SINK | TRB-3 | TRIB3 | TRB3 | DJ1103G7.3 | SKIP3 | Tribbles homolog 3
- Origin Species
- Human
- NCBI GenBank
- NM_021158
- SwissProt
- Q96RU7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRATPLAAPA GSLSRKKRLE LDDNLDTERP VQKRARSGPQ PRLPPCLLPL SPPTAPDRAT61AVATASRLGP YVLLEPEEGG RAYQALHCPT GTEYTCKVYP VQEALAVLEP YARLPPHKHV121ARPTEVLAGT QLLYAFFTRT HGDMHSLVRS RHRIPEPEAA VLFRQMATAL AHCHQHGLVL181RDLKLCRFVF ADRERKKLVL ENLEDSCVLT GPDDSLWDKH ACPAYVGPEI LSSRASYSGK241AADVWSLGVA LFTMLAGHYP FQDSEPVLLF GKIRRGAYAL PAGLSAPARC LVRCLLRREP301AERLTATGIL LHPWLRQDPM PLAPTRSHLW EAAQVVPDGL GLDEAREEEG DREVVLYGTR361RLEQKLISEEDLAANDILDYKDDDDKV
361 aa + 26 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.