Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRAFD1 / FLN29 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100889-100
Catalog Number: LS-G100889
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRAFD1 (NM_006700), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FLN29 | Protein FLN29 | TRAFD1 | FLN29 gene product
- Origin Species
- Human
- NCBI GenBank
- NM_006700
- SwissProt
- O14545
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEFLDDQET RLCDNCKKEI PVFNFTIHEI HCQRNIGMCP TCKEPFPKSD METHMAAEHC61QVTCKCNKKL EKRLLKKHEE TECPLRLAVC QHCDLELSIL KLKEHEDYCG ARTELCGNCG121RNVLVKDLKT HPEVCGREGE EKRNEVAIPP NAYDESWGQD GIWIASQLLR QIEALDPPMR181LPRRPLRAFE SDVFHNRTTN QRNITAQVSI QNNLFEEQER QERNRGQQPP KEGGEESANL241DFMLALSLQN EGQASSVAEQ DFWRAVCEAD QSHGGPRSLS DIKGAADEIM LPCEFCEELY301PEELLIDHQT SCNPSRALPS LNTGSSSPRG VEEPDVIFQN FLQQAASNQL DSLMGLSNSH361PVEESIIIPC EFCGVQLEEE VLFHHQDQCD QRPATATNHV TEGIPRLDSQ PQETSPELPR421RRVRHQGDLS SGYLDDTKQE TANGPTSCLP PSRPINNMTA TYNQLSRSTS GPRPGCQPSS481PCVPKLSNSD SQDIQGRNRD SQNGAIAPGH VSVIRPPQNL YPENIVPSFS PGPSGRYGAS541GRSEGGRNSR VTPAAANYRS RTAKAKPSKQ QGAGDAEEEE EETRTRPLEQKLISEEDLAANDILDYKDDDDKV
582 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.