LSBio
Human TRAFD1 / FLN29 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TRAFD1 / FLN29 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all TRAFD1 antibodies →
$153.00each
Catalog Number: LS-G79840-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TRAFD1 (NM_001143906) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FLN29 | Protein FLN29 | TRAFD1 | FLN29 gene product
Origin Species
Human
NCBI GenBank
NM_006700
SwissProt
O14545

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEFLDDQET RLCDNCKKEI PVFNFTIHEI HCQRNIGMCP TCKEPFPKSD METHMAAEHC
61QVTCKCNKKL EKRLLKKHEE TECPLRLAVC QHCDLELSIL KLKEHEDYCG ARTELCGNCG
121RNVLVKDLKT HPEVCGREGE EKRNEVAIPP NAYDESWGQD GIWIASQLLR QIEALDPPMR
181LPRRPLRAFE SDVFHNRTTN QRNITAQVSI QNNLFEEQER QERNRGQQPP KEGGEESANL
241DFMLALSLQN EGQASSVAEQ DFWRAVCEAD QSHGGPRSLS DIKGAADEIM LPCEFCEELY
301PEELLIDHQT SCNPSRALPS LNTGSSSPRG VEEPDVIFQN FLQQAASNQL DSLMGLSNSH
361PVEESIIIPC EFCGVQLEEE VLFHHQDQCD QRPATATNHV TEGIPRLDSQ PQETSPELPR
421RRVRHQGDLS SGYLDDTKQE TANGPTSCLP PSRPINNMTA TYNQLSRSTS GPRPGCQPSS
481PCVPKLSNSD SQDIQGRNRD SQNGAIAPGH VSVIRPPQNL YPENIVPSFS PGPSGRYGAS
541GRSEGGRNSR VTPAAANYRS RTAKAKPSKQ QGAGDAEEEE EE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

582 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.