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TPTE Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TPTE Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G103368-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TPTE (NM_199259), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 44 | CT44 | TPTE | PTEN2 | Tumor antigen BJ-HCC-5
Origin Species
Human
NCBI GenBank
NM_199261
SwissProt
P56180

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNESPDPTDL AGVIIELGPN DSPQTSEFKG ATEEAPAKES VLARLSKFEV EDAENVASYD
61SKIKKIVHSI VSSFAFGLFG VFLVLLDVTL ILADLIFTDS KLYIPLEYRS ISLAIALFFL
121MDVLLRVFVE RRQQYFSDLF NILDTAIIVI LLLVDVVYIF FDIKLLRNIP RWTHLLRLLR
181LIILLRIFHL FHQKRQLEKL IRRRVSENKR RYTRDGFDLD LTYVTERIIA MSFPSSGRQS
241FYRNPIKEVV RFLDKKHRNH YRVYNLCSER AYDPKHFHNR VVRIMIDDHN VPTLHQMVVF
301TKEVNEWMAQ DLENIVAIHC KGGTDRTGTM VCAFLIASEI CSTAKESLYY FGERRTDKTH
361SEKFQGVETP SQKRYVAYFA QVKHLYNWNL PPRRILFIKH FIIYSIPRYV RDLKIQIEME
421KKVVFSTISL GKCSVLDNIT TDKILIDVFD GPPLYDDVKV QFFYSNLPTY YDNCSFYFWL
481HTSFIENNRL YLPKNELDNL HKQKARRIYP SDFAVEILFG EKMTSSDVVA GSD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

533 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.