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TPTE Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TPTE Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TPTE antibodies →
$725.00each
Catalog Number: LS-G103367-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TPTE (NM_199260), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 44 | CT44 | TPTE | PTEN2 | Tumor antigen BJ-HCC-5
Origin Species
Human
NCBI GenBank
NM_199261
SwissProt
P56180

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60 kda
Protein Type
Lysate
Amino Acid Sequence
1MNESPDPTDL AGVIIELGPN DSPQTSEFKG ATEEAPAKES SKIKKIVHSI VSSFAFGLFG
61VFLVLLDVTL ILADLIFTDS KLYIPLEYRS ISLAIALFFL MDVLLRVFVE RRQQYFSDLF
121NILDTAIIVI LLLVDVVYIF FDIKLLRNIP RWTHLLRLLR LIILLRIFHL FHQKRQLEKL
181IRRRVSENKR RYTRDGFDLD LTYVTERIIA MSFPSSGRQS FYRNPIKEVV RFLDKKHRNH
241YRVYNLCSER AYDPKHFHNR VVRIMIDDHN VPTLHQMVVF TKEVNEWMAQ DLENIVAIHC
301KGGTDRTGTM VCAFLIASEI CSTAKESLYY FGERRTDKTH SEKFQGVETP SQKRYVAYFA
361QVKHLYNWNL PPRRILFIKH FIIYSIPRYV RDLKIQIEME KKVVFSTISL GKCSVLDNIT
421TDKILIDVFD GPPLYDDVKV QFFYSNLPTY YDNCSFYFWL HTSFIENNRL YLPKNELDNL
481HKQKARRIYP SDFAVEILFG EKMTSSDVVA GSD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

513 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.