Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TPC1 / TPCN1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G126199-100
Catalog Number: LS-G126199
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TPCN1 (NM_001143819), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1169 | Two-pore segment channel 1 | TPC1 | TPCN1 | Two pore segment channel 1 | Two-pore channel 1
- Origin Species
- Human
- NCBI GenBank
- AX235888
- SwissProt
- Q9ULQ1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESCYIAQAG LELLGSSSSP TLTSQSAEIT EDASNGGVSE QHPWPSGFER ELKPETISSP61GYHILRATGE ENMAVSLDDD VPLILTLDEG GSAPLAPSNG LGQEELPSKN GGSYAIHDSQ121APSLSSGGES SPSSPAHNWE MNYQEAAIYL QEGENNDKFF THPKDAKALA AYLFAHNHLF181YLMELATALL LLLLSLCEAP AVPALRLGIY VHATLELFAL MVVVFELCMK LRWLGLHTFI241RHKRTMVKTS VLVVQFVEAI VVLVRQMSHV RVTRALRCIF LVDCRYCGGV RRNLRQIFQS301LPPFMDILLL LLFFMIIFAI LGFYLFSPNP SDPYFSTLEN SIVSLFVLLT TANFPDVMMP361SYSRNPWSCV FFIVYLSIEL YFIMNLLLAV VFDTFNDIEK RKFKSLLLHK RTAIQHAYRL421LISQRRPAGI SYRQFEGLMR FYKPRMSARE RYLTFKALNQ NNTPLLSLKD FYDIYEVAAL481KWKAKKNREH WFDELPRTAL LIFKGINILV KSKAFQYFMY LVVAVNGVWI LVETFMLKGG541NFFSKHVPWS YLVFLTIYGV ELFLKVAGLG PVEYLSSGWN LFDFSVTVFA FLGLLALALN601MEPFYFIVVL RPLQLLRLFK LKERYRNVLD TMFELLPRMA SLGLTLLIFY YSFAIVGMEF661FCGIVFPNCC NTSTVADAYR WRNHTVGNRT VVEEGYYYLN NFDNILNSFV TLFELTVVNN721WYIIMEGVTS QTSHWSRLYF MTFYIVTMVV MTIIVAFILE AFVFRMNYSR KNQDSEVDGG781ITLEKEISKE ELVAVLELYR EARGASSDVT RLLETLSQME RYQQHSMVFL GRRSRTKSDL841SLKMYQEEIQ EWYEEHAREQ EQQRQLSSSA APAAQQPPGS RQRSQTVTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
888 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.