LSBio
Human TPC1 / TPCN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TPC1 / TPCN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all TPCN1 antibodies →
$220.00each
Catalog Number: LS-G85603-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TPCN1 (NM_001143819) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1169 | Two-pore segment channel 1 | TPC1 | TPCN1 | Two pore segment channel 1 | Two-pore channel 1
Origin Species
Human
NCBI GenBank
AX235888
SwissProt
Q9ULQ1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESCYIAQAG LELLGSSSSP TLTSQSAEIT EDASNGGVSE QHPWPSGFER ELKPETISSP
61GYHILRATGE ENMAVSLDDD VPLILTLDEG GSAPLAPSNG LGQEELPSKN GGSYAIHDSQ
121APSLSSGGES SPSSPAHNWE MNYQEAAIYL QEGENNDKFF THPKDAKALA AYLFAHNHLF
181YLMELATALL LLLLSLCEAP AVPALRLGIY VHATLELFAL MVVVFELCMK LRWLGLHTFI
241RHKRTMVKTS VLVVQFVEAI VVLVRQMSHV RVTRALRCIF LVDCRYCGGV RRNLRQIFQS
301LPPFMDILLL LLFFMIIFAI LGFYLFSPNP SDPYFSTLEN SIVSLFVLLT TANFPDVMMP
361SYSRNPWSCV FFIVYLSIEL YFIMNLLLAV VFDTFNDIEK RKFKSLLLHK RTAIQHAYRL
421LISQRRPAGI SYRQFEGLMR FYKPRMSARE RYLTFKALNQ NNTPLLSLKD FYDIYEVAAL
481KWKAKKNREH WFDELPRTAL LIFKGINILV KSKAFQYFMY LVVAVNGVWI LVETFMLKGG
541NFFSKHVPWS YLVFLTIYGV ELFLKVAGLG PVEYLSSGWN LFDFSVTVFA FLGLLALALN
601MEPFYFIVVL RPLQLLRLFK LKERYRNVLD TMFELLPRMA SLGLTLLIFY YSFAIVGMEF
661FCGIVFPNCC NTSTVADAYR WRNHTVGNRT VVEEGYYYLN NFDNILNSFV TLFELTVVNN
721WYIIMEGVTS QTSHWSRLYF MTFYIVTMVV MTIIVAFILE AFVFRMNYSR KNQDSEVDGG
781ITLEKEISKE ELVAVLELYR EARGASSDVT RLLETLSQME RYQQHSMVFL GRRSRTKSDL
841SLKMYQEEIQ EWYEEHAREQ EQQRQLSSSA APAAQQPPGS RQRSQTVT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

888 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.