Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TP1 / TEP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114854-100
Catalog Number: LS-G114854
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TEP1 (NM_007110), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- p80 telomerase homolog | TEP1 | Telomerase protein 1 | TLP1 | TROVE domain family | member 1 | TROVE1 | VAULT2 | p240 | Telomerase protein component 1 | TP1
- Origin Species
- Human
- NCBI GenBank
- NM_007110
- SwissProt
- Q99973
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 290.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKLHGHVSA HPDILSLENR CLAMLPDLQP LEKLHQHVST HSDILSLKNQ CLATLPDLKT61MEKPHGYVSA HPDILSLENQ CLATLSDLKT MEKPHGHVSA HPDILSLENR CLATLSSLKS121TVSASPLFQS LQISHMTQAD LYRVNNSNCL LSEPPSWRAQ HFSKGLDLST CPIALKSISA181TETAQEATLG RWFDSEEKKG AETQMPSYSL SLGEEEEVED LAVKLTSGDS ESHPEPTDHV241LQEKKMALLS LLCSTLVSEV NMNNTSDPTL AAIFEICREL ALLEPEFILK ASLYARQQLN301VRNVANNILA IAAFLPACRP HLRRYFCAIV QLPSDWIQVA ELYQSLAEGD KNKLVPLPAC361LRTAMTDKFA QFDEYQLAKY NPRKHRAKRH PRRPPRSPGM EPPFSHRCFP RYIGFLREEQ421RKFEKAGDTV SEKKNPPRFT LKKLVQRLHI HKPAQHVQAL LGYRYPSNLQ LFSRSRLPGP481WDSSRAGKRM KLSRPETWER ELSLRGNKAS VWEELIENGK LPFMAMLRNL CNLLRVGISS541RHHELILQRL QHAKSVIHSR QFPFRFLNAH DAIDALEAQL RNQALPFPSN ITLMRRILTR601NEKNRPRRRF LCHLSRQQLR MAMRIPVLYE QLKREKLRVH KARQWKYDGE MLNRYRQALE661TAVNLSVKHS LPLLPGRTVL VYLTDANADR LCPKSNPQGP PLNYALLLIG MMITRAEQVD721VVLCGGDTLK TAVLKAEEGI LKTAIKLQAQ VQEFDENDGW SLNTFGKYLL SLAGQRVPVD781RVILLGQSMD DGMINVAKQL YWQRVNSKCL FVGILLRRVQ YLSTDLNPND VTLSGCTDAI841LKFIAEHGAS HLLEHVGQMD KIFKIPPPPG KTGVQSLRPL EEDTPSPLAP VSQQGWRSIR901LFISSTFRDM HGERDLLLRS VLPALQARAA PHRISLHGID LRWGVTEEET RRNRQLEVCL961GEVENAQLFV GILGSRYGYI PPSYNLPDHP HFHWAQQYPS GRSVTEMEVM QFLNRNQRLQ1021PSAQALIYFR DSSFLSSVPD AWKSDFVSES EEAARRISEL KSYLSRQKGI TCRRYPCEWG1081GVAAGRPYVG GLEEFGQLVL QDVWNMIQKL YLQPGALLEQ PVSIPDDDLV QATFQQLQKP1141PSPARPRLLQ DTVQRLMLPH GRLSLVTGQS GQGKTAFLAS LVSALQAPDG AKVASLVFFH1201FSGARPDQGL ALTLLRRLCT YLRGQLKEPG ALPSTYRSLV WELQQRLLPK SAESLHPGQT1261QVLIIDGADR LVDQNGQLIS DWIPKKLPRC VHLVLSVSSD AGLGETLEQS QGAHVLALGP1321LEASARARLV REELALYGKR LEESPFNNQM RLLLVKRESG RPLYLRLVTD HLRLFTLYEQ1381VSERLRTLPA TVPLLLQHIL STLEKEHGPD VLPQALTALE VTRSGLTVDQ LHGVLSVWRT1441LPKGTKSWEE AVAAGNSGDP YPMGPFACLV QSLRSLLGEG PLERPGARLC LPDGPLRTAA1501KRCYGKRPGL EDTAHILIAA QLWKTCDADA SGTFRSCPPE ALGDLPYHLL QSGNRGLLSK1561FLTNLHVVAA HLELGLVSRL LEAHALYASS VPKEEQKLPE ADVAVFRTFL RQQASILSQY1621PRLLPQQAAN QPLDSPLCHQ ASLLSRRWHL QHTLRWLNKP RTMKNQQSSS LSLAVSSSPT1681AVAFSTNGQR AAVGTANGTV YLLDLRTWQE EKSVVSGCDG ISACLFLSDD TLFLTAFDGL1741LELWDLQHGC RVLQTKAHQY QITGCCLSPD CRLLATVCLG GCLKLWDTVR GQLAFQHTYP1801KSLNCVAFHP EGQVIATGSW AGSISFFQVD GLKVTKDLGA PGASIRTLAF NVPGGVVAVG1861RLDSMVELWA WREGARLAAF PAHHGFVAAA LFLHAGCQLL TAGEDGKVQV WSGSLGRPRG1921HLGSLSLSPA LSVALSPDGD RVAVGYRADG IRIYKISSGS QGAQGQALDV AVSALAWLSP1981KVLVSGAEDG SLQGWALKEC SLQSLWLLSR FQKPVLGLAT SQELLASASE DFTVQLWPRQ2041LLTRPHKAED FPCGTELRGH EGPVSCCSFS TDGGSLATGG RDRSLLCWDV RTPKTPVLIH2101SFPACHRDWV TGCAWTKDNL LISCSSDGSV GLWDPESGQR LGQFLGHQSA VSAVAAVEEH2161VVSVSRDGTL KVWDHQGVEL TSIPAHSGPI SHCAAAMEPR AAGQPGSELL VVTVGLDGAT2221RLWHPLLVCQ THTLLGHSGP VRAAAVSETS GLMLTASEDG SVRLWQVPKE ADDTCIPRSS2281AAVTAVAWAP DGSMAVSGNQ AGELILWQEA KAVATAQAPG HIGALIWSSA HTFFVLSADE2341KISEWQVKLR KGSAPGNLSL HLNRILQEDL GVLTSLDWAP DGHFLILAKA DLKLLCMKPG2401DAPSEIWSSY TENPMILSTH KEYGIFVLQP KDPGVLSFLR QKESGEFEER LNFDINLENP2461SRTLISITQA KPESESSFLC ASSDGILWNL AKCSPEGEWT TGNMWQKKAN TPETQTPGTD2521PSTCRESDAS MDSDASMDSE PTPHLKTRQR RKIHSGSVTA LHVLPELLVT ASKDRDVKLW2581ERPSMQLLGL FRCEGSVSCL EPWLGANSTL QLAVGDVQGN VYFLNWETRTRPLEQKLISEEDLAANDILDYKDDDDKV
2627 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.