LSBio
TOX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TOX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TOX2 antibodies →
$725.00each
Catalog Number: LS-G118979-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TOX2 (NM_001098798), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ1108D11.2 | DJ495O3.1 | Granulosa cell HMG box 1 | TOX2 | C20orf100 | GCX-1 | GCX1
Origin Species
Human
NCBI GenBank
NM_032883
SwissProt
Q96NM4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQQTRTEAVA GAFSRCLGFC GMRLGLLLLA RHWCIAGVFP QKFDGDSAYV GMSDGNPELL
61STSQTYNGQS ENNEDYEIPP ITPPNLPEPS LLHLGDHEAS YHSLCHGLTP NGLLPAYSYQ
121AMDLPAIMVS NMLAQDSHLL SGQLPTIQEM VHSEVAAYDS GRPGPLLGRP AMLASHMSAL
181SQSQLISQMG IRSSIAHSSP SPPGSKSATP SPSSSTQEEE SEVHFKISGE KRPSADPGKK
241AKNPKKKKKK DPNEPQKPVS AYALFFRDTQ AAIKGQNPSA TFGDVSKIVA SMWDSLGEEQ
301KQSSPDQGET KSTQANPPAK MLPPKQPMYA MPGLASFLTP SDLQAFRSGA SPASLARTLG
361SKSLLPGLSA SPPPPPSFPL SPTLHQQLSL PPHAQGALLS PPVSMSPAPQ PPVLPTPMAL
421QVQLAMSPSP PGPQDFPHIS EFPSSSGSCS PGPSNPTSSG DWDSSYPSGE CGISTCSLLP
481RDKSLYLT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

488 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.