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TOX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TOX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TOX2 antibodies →
$725.00each
Catalog Number: LS-G118976-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TOX2 (NM_001098796), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ1108D11.2 | DJ495O3.1 | Granulosa cell HMG box 1 | TOX2 | C20orf100 | GCX-1 | GCX1
Origin Species
Human
NCBI GenBank
NM_032883
SwissProt
Q96NM4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDGNPELLS TSQTYNGQSE NNEDYEIPPI TPPNLPEPSL LHLGDHEASY HSLCHGLTPN
61GLLPAYSYQA MDLPAIMVSN MLAQDSHLLS GQLPTIQEMV HSEVAAYDSG RPGPLLGRPA
121MLASHMSALS QSQLISQMGI RSSIAHSSPS PPGSKSATPS PSSSTQEEES EVHFKISGEK
181RPSADPGKKA KNPKKKKKKD PNEPQKPVSA YALFFRDTQA AIKGQNPSAT FGDVSKIVAS
241MWDSLGEEQK QAYKRKTEAA KKEYLKALAA YRASLVSKSS PDQGETKSTQ ANPPAKMLPP
301KQPMYAMPGL ASFLTPSDLQ AFRSGASPAS LARTLGSKSL LPGLSASPPP PPSFPLSPTL
361HQQLSLPPHA QGALLSPPVS MSPAPQPPVL PTPMALQVQL AMSPSPPGPQ DFPHISEFPS
421SSGSCSPGPS NPTSSGDWDS SYPSGECGIS TCSLLPRDKS LYLT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

464 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.