Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TMEM16B / ANO2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111198-100
Catalog Number: LS-G111198
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TMEM16B(ANO2) (NM_020373), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Anoctamin 2 | Anoctamin-2 | C12orf3 | Transmembrane protein 16B | ANO2 | TMEM16B
- Origin Species
- Human
- NCBI GenBank
- NM_020373
- SwissProt
- Q9NQ90
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 113.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPEDIPLLPG SPRRLSPQAG SRGGQGPKHG QQCLKMPGPR APGLQGGSNR DPGQPCGGES61TRSSSVINNY LDANEPVSLE ARLSRMHFHD SQRKVDYVLA YHYRKRGVHL AQGFPGHSLA121IVSNGETGKE PHAGGPGDIE LGPLDALEEE RKEQREEFEH NLMEAGLELE KDLENKSQGS181IFVRIHAPWQ VLAREAEFLK IKVPTKKEMY EIKAGGSIAK KFSAALQKLS SHLQPRVPEH241SNNKMKNLSY PFSREKMYLY NIQEKDTFFD NATRSRIVHE ILKRTACSRA NNTMGINSLI301ANNIYEAAYP LHDGEYDSPE DDMNDRKLLY QEWARYGVFY KFQPIDLIRK YFGEKIGLYF361AWLGLYTSFL IPSSVIGVIV FLYGCATIEE DIPSREMCDQ QNAFTMCPLC DKSCDYWNLS421SACGTAQASH LFDNPATVFF SIFMALWATM FLENWKRLQM RLGYFWDLTG IEEEEERAQE481HSRPEYETKV REKMLKESNQ SAVQKLETNT TECGDEDDED KLTWKDRFPG YLMNFASILF541MIALTFSIVF GVIVYRITTA AALSLNKATR SNVRVTVTAT AVIINLVVIL ILDEIYGAVA601KWLTKIEVPK TEQTFEERLI LKAFLLKFVN AYSPIFYVAF FKGRFVGRPG SYVYVFDGYR661MEECAPGGCL MELCIQLSII MLGKQLIQNN IFEIGVPKLK KLFRKLKDET EAGETDSAHS721KHPEQWDLDY SLEPYTGLTP EYMEMIIQFG FVTLFVASFP LAPVFALLNN VIEVRLDAKK781FVTELRRPDA VRTKDIGIWF DILSGIGKFS VISNAFVIAI TSDFIPRLVY QYSYSHNGTL841HGFVNHTLSF FNVSQLKEGT QPENSQFDQE VQFCRFKDYR EPPWAPNPYE FSKQYWFILS901ARLAFVIIFQ NLVMFLSVLV DWMIPDIPTD ISDQIKKEKS LLVDFFLKEE HEKLKLMDEP961ALRSPGGGDR SRSRAASSAP SGQSQLGSMM SSGSQHTNVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
999 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.