LSBio
Human TMEM16B / ANO2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TMEM16B / ANO2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ANO2 antibodies →
$220.00each
Catalog Number: LS-G91244-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TMEM16B(ANO2) (NM_020373) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Anoctamin 2 | Anoctamin-2 | C12orf3 | Transmembrane protein 16B | ANO2 | TMEM16B
Origin Species
Human
NCBI GenBank
NM_020373
SwissProt
Q9NQ90

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPEDIPLLPG SPRRLSPQAG SRGGQGPKHG QQCLKMPGPR APGLQGGSNR DPGQPCGGES
61TRSSSVINNY LDANEPVSLE ARLSRMHFHD SQRKVDYVLA YHYRKRGVHL AQGFPGHSLA
121IVSNGETGKE PHAGGPGDIE LGPLDALEEE RKEQREEFEH NLMEAGLELE KDLENKSQGS
181IFVRIHAPWQ VLAREAEFLK IKVPTKKEMY EIKAGGSIAK KFSAALQKLS SHLQPRVPEH
241SNNKMKNLSY PFSREKMYLY NIQEKDTFFD NATRSRIVHE ILKRTACSRA NNTMGINSLI
301ANNIYEAAYP LHDGEYDSPE DDMNDRKLLY QEWARYGVFY KFQPIDLIRK YFGEKIGLYF
361AWLGLYTSFL IPSSVIGVIV FLYGCATIEE DIPSREMCDQ QNAFTMCPLC DKSCDYWNLS
421SACGTAQASH LFDNPATVFF SIFMALWATM FLENWKRLQM RLGYFWDLTG IEEEEERAQE
481HSRPEYETKV REKMLKESNQ SAVQKLETNT TECGDEDDED KLTWKDRFPG YLMNFASILF
541MIALTFSIVF GVIVYRITTA AALSLNKATR SNVRVTVTAT AVIINLVVIL ILDEIYGAVA
601KWLTKIEVPK TEQTFEERLI LKAFLLKFVN AYSPIFYVAF FKGRFVGRPG SYVYVFDGYR
661MEECAPGGCL MELCIQLSII MLGKQLIQNN IFEIGVPKLK KLFRKLKDET EAGETDSAHS
721KHPEQWDLDY SLEPYTGLTP EYMEMIIQFG FVTLFVASFP LAPVFALLNN VIEVRLDAKK
781FVTELRRPDA VRTKDIGIWF DILSGIGKFS VISNAFVIAI TSDFIPRLVY QYSYSHNGTL
841HGFVNHTLSF FNVSQLKEGT QPENSQFDQE VQFCRFKDYR EPPWAPNPYE FSKQYWFILS
901ARLAFVIIFQ NLVMFLSVLV DWMIPDIPTD ISDQIKKEKS LLVDFFLKEE HEKLKLMDEP
961ALRSPGGGDR SRSRAASSAP SGQSQLGSMM SSGSQHTNV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

999 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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