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TLE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TLE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126341-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TLE2 (NM_001144762), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ESG | Enhancer of split groucho 2 | ESG2 | GRG2 | TLE2
Origin Species
Human
NCBI GenBank
NM_003260
SwissProt
Q04725

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQRHYVMYYE MSYGLNIEMH KQAEIVKRLS GICAQIIPFL TQEHQQQVLQ AVERAKQVTV
61GELNSLIGSA SPSPPESLVE EERPSGPGGG GKQRADEKEP SGPYESDEDK SDYNLVVDED
121QPSEPPSPAT TPCGKVPICI PARRDLVDSP ASLASSLGSP LPRAKELILN DLPASTPASK
181SCDSSPPQDA STPGPSSASH LCQLAAKPAP STDSVALRSP LTLSSPFTTS FSLGSHSTLN
241GDLSVPSSYV SLHLSPQVSS SVVYGRSPVM AFESHPHLRG SSVSSSLPSI PGGKPAYSFH
301VSADGQMQPV PFPSDALVGA GIPRHARQLH TLAHGEVVCA VTISGSTQHV YTGGKGCVKV
361WDVGQPGAKT PVAQLDCLNR DNYIRSCKLL PDGRSLIVGG EASTLSIWDL AAPTPRIKAE
421LTSSAPACYA LAVSPDAKVC FSCCSDGNIV VWDLQNQTMV RQFQGHTDGA SCIDISDYGT
481RLWTGGLDNT VRCWDLREGR QLQQHDFSSQ IFSLGHCPNQ DWLAVGMESS NVEILHVRKP
541EKYQLHLHES CVLSLKFASC GRWFVSTGKD NLLNAWRTPY GASIFQSKES SSVLSCDISR
601NNKYIVTGSG DKKATVYEVV Y
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

621 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.