TLE2 Antibody
Catalog Number: LS-C809263
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human TLE2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G87963-20
Catalog Number: LS-G87963
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human TLE2 (NM_001144762) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ESG | Enhancer of split groucho 2 | ESG2 | GRG2 | TLE2
- Origin Species
- Human
- NCBI GenBank
- NM_003260
- SwissProt
- Q04725
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQRHYVMYYE MSYGLNIEMH KQAEIVKRLS GICAQIIPFL TQEHQQQVLQ AVERAKQVTV61GELNSLIGSA SPSPPESLVE EERPSGPGGG GKQRADEKEP SGPYESDEDK SDYNLVVDED121QPSEPPSPAT TPCGKVPICI PARRDLVDSP ASLASSLGSP LPRAKELILN DLPASTPASK181SCDSSPPQDA STPGPSSASH LCQLAAKPAP STDSVALRSP LTLSSPFTTS FSLGSHSTLN241GDLSVPSSYV SLHLSPQVSS SVVYGRSPVM AFESHPHLRG SSVSSSLPSI PGGKPAYSFH301VSADGQMQPV PFPSDALVGA GIPRHARQLH TLAHGEVVCA VTISGSTQHV YTGGKGCVKV361WDVGQPGAKT PVAQLDCLNR DNYIRSCKLL PDGRSLIVGG EASTLSIWDL AAPTPRIKAE421LTSSAPACYA LAVSPDAKVC FSCCSDGNIV VWDLQNQTMV RQFQGHTDGA SCIDISDYGT481RLWTGGLDNT VRCWDLREGR QLQQHDFSSQ IFSLGHCPNQ DWLAVGMESS NVEILHVRKP541EKYQLHLHES CVLSLKFASC GRWFVSTGKD NLLNAWRTPY GASIFQSKES SSVLSCDISR601NNKYIVTGSG DKKATVYEVV YTRTRPLEQKLISEEDLAANDILDYKDDDDKV
621 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
