Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TJP3 / ZO3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113943-100
Catalog Number: LS-G113943
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Zonula occludens protein 3(TJP3) (NM_014428), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Tight junction protein 3 | TJP3 | ZO-3 | Zona occludens protein 3 | Zonula occludens protein 3 | Tight junction protein ZO-3 | ZO3
- Origin Species
- Human
- NCBI GenBank
- NM_014428
- SwissProt
- O95049
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 104.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNLCGLMPIF PAPLDQVADM EELTIWEQHT ATLSKDPRRG FGIAISGGRD RPGGSMVVSD61VVPGGPAEGR LQTGDHIVMV NGVSMENATS AFAIQILKTC TKMANITVKR PRRIHLPATK121ASPSSPGRQD SDEDDGPQRV EEVDQGRGYD GDSSSGSGRS WDERSRRPRP GRRGRAGSHG181RRSPGGGSEA NGLALVSGFK RLPRQDVQMK PVKSVLVKRR DSEEFGVKLG SQIFIKHITD241SGLAARHRGL QEGDLILQIN GVSSQNLSLN DTRRLIEKSE GKLSLLVLRD RGQFLVNIPP301AVSDSDSSPL EEGVTMADEM SSPPADISDL ASELSQAPPS HIPPPPRHAQ RSPEASQTDS361PVESPRLRRE SSVDSRTISE PDEQRSELPR ESSYDIYRVP SSQSMEDRGY SPDTRVVRFL421KGKSIGLRLA GGNDVGIFVS GVQAGSPADG QGIQEGDQIL QVNDVPFQNL TREEAVQFLL481GLPPGEEMEL VTQRKQDIFW KMVQSRVGDS FYIRTHFELE PSPPSGLGFT RGDVFHVLDT541LHPGPGQSHA RGGHWLAVRM GRDLREQERG IIPNQSRAEQ LASLEAAQRA VGVGPGSSAG601SNARAEFWRL RGLRRGAKKT TQRSREDLSA LTRQGRYPPY ERVVLREASF KRPVVILGPV661ADIAMQKLTA EMPDQFEIAE TVSRTDSPSK IIKLDTVRVI AEKDKHALLD VTPSAIERLN721YVQYYPIVVF FIPESRPALK ALRQWLAPAS RRSTRRLYAQ AQKLRKHSSH LFTATIPLNG781TSDTWYQELK AIIREQQTRP IWTAEDQLDG SLEDNLDLPH HGLADSSADL SCDSRVNSDY841ETDGEGGAYT DGEGYTDGEG GPYTDVDDEP PAPALARSSE PVQADESQSP RDRGRISAHQ901GAQVDSRHPQ GQWRQDSMRT YEREALKKKF MRVHDAESSD EDGYDWGPAT DLSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
952 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.