Detection of human TJP3/ZO-3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TJP3/ZO-3 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
TJP3 / ZO3 Antibody (aa883-933)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$503.00each
Catalog Number: LS-C762446-100
Catalog Number: LS-C762446
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- Description
- ZO3 antibody LS-C762446 is an unconjugated rabbit polyclonal antibody to human ZO3 (TJP3) (aa883-933). Validated for IP and WB.
- Application
- IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Dog, Mouse
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Tight junction protein 3 | TJP3 | ZO-3 | Zona occludens protein 3 | Zonula occludens protein 3 | Tight junction protein ZO-3 | ZO3
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- O95049
- NCBI GenBank
- NM_014428 | NP_055243.1
- SwissProt
- O95049
Target
- Target
- Human TJP3 / ZO3
- Antigen Species
- Human
- Gene Name
- TJP3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A portion of GeneID: 27134 corresponding to ammino acids between 883 and 933 of UniprotID: O95049
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 year.
- Recommended Storage Buffer
- Tris-citrate