Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TGFB4 / LEFTY2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129635-100
Catalog Number: LS-G129635
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LEFTY2 (NM_001172425), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EBAF | LEFTYA | Protein lefty-2 | LEFTA | LEFTY2 | TGFB4 | Protein lefty-A | TGF-beta-4
- Origin Species
- Human
- NCBI GenBank
- NM_003240
- SwissProt
- O00292
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWPLWLCWAL WVLPLAGPGA ALTEEQLLGS LLRQLQLSEV PVLDRADMEK LVIPAHVRAQ61YVVLLRRSHG DRSRGKRFSQ SFREVAGRFL ASEAALHRHG RLSPRSAQAR VTVEWLRVRD121DGSNRTSLID SRLVSVHESG WKAFDVTEAV NFWQQLSRPR QPLLLQVSVQ REHLGPLASG181AHKLVRFASQ GAPAGLGEPQ LELHTLDLRD YGAQGDCDPE APMTEGTRCC RQEMYIDLQG241MKWAKNWVLE PPGFLAYECV GTCQQPPEAL AFNWPFLGPR QCIASETASL PMIVSIKEGG301RTRPQVVSLP NMRVQKCSCA SDGALVPRRL QPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
332 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.