Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TFAP2A / AP-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119504-100
Catalog Number: LS-G119504
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AP2 alpha(TFAP2A) (NM_001042425), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Activator protein 2 | AP-2 | AP-2a | AP2TF | AP-2alpha | BOFS | TFAP2 | TFAP2A | Transcription factor AP-2 | AP-2 transcription factor | AP2-alpha
- Origin Species
- Human
- NCBI GenBank
- NM_003220
- SwissProt
- P05549
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSILAKMGDW QDRHDGTSNG TARLPQLGTV GQSPYTSAPP LSHTPNADFQ PPYFPPPYQP61IYPQSQDPYS HVNDPYSLNP LHAQPQPQHP GWPGQRQSQE SGLLHTHRGL PHQLSGLDPR121RDYRRHEDLL HGPHALSSGL GDLSIHSLPH AIEEVPHVED PGINIPDQTV IKKGPVSLSK181SNSNAVSAIP INKDNLFGGV VNPNEVFCSV PGRLSLLSST SKYKVTVAEV QRRLSPPECL241NASLLGGVLR RAKSKNGGRS LREKLDKIGL NLPAGRRKAA NVTLLTSLVE GEAVHLARDF301GYVCETEFPA KAVAEFLNRQ HSDPNEQVTR KNMLLATKQI CKEFTDLLAQ DRSPLGNSRP361NPILEPGIQS CLTHFNLISH GFGSPAVCAA VTALQNYLTE ALKAMDKMYL SNNPNSHTDN421NAKSSDKEEK HRKSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
433 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.