TFAP2A / AP-2 Antibody
Catalog Number: LS-C496655
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human TFAP2A / AP-2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G79210-20
Catalog Number: LS-G79210
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AP2 alpha(TFAP2A) (NM_001042425) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Activator protein 2 | AP-2 | AP-2a | AP2TF | AP-2alpha | BOFS | TFAP2 | TFAP2A | Transcription factor AP-2 | AP-2 transcription factor | AP2-alpha
- Origin Species
- Human
- NCBI GenBank
- NM_003220
- SwissProt
- P05549
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSILAKMGDW QDRHDGTSNG TARLPQLGTV GQSPYTSAPP LSHTPNADFQ PPYFPPPYQP61IYPQSQDPYS HVNDPYSLNP LHAQPQPQHP GWPGQRQSQE SGLLHTHRGL PHQLSGLDPR121RDYRRHEDLL HGPHALSSGL GDLSIHSLPH AIEEVPHVED PGINIPDQTV IKKGPVSLSK181SNSNAVSAIP INKDNLFGGV VNPNEVFCSV PGRLSLLSST SKYKVTVAEV QRRLSPPECL241NASLLGGVLR RAKSKNGGRS LREKLDKIGL NLPAGRRKAA NVTLLTSLVE GEAVHLARDF301GYVCETEFPA KAVAEFLNRQ HSDPNEQVTR KNMLLATKQI CKEFTDLLAQ DRSPLGNSRP361NPILEPGIQS CLTHFNLISH GFGSPAVCAA VTALQNYLTE ALKAMDKMYL SNNPNSHTDN421NAKSSDKEEK HRKSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
433 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
