LSBio
Human TFAP2A / AP-2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TFAP2A / AP-2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G79210-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AP2 alpha(TFAP2A) (NM_001042425) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Activator protein 2 | AP-2 | AP-2a | AP2TF | AP-2alpha | BOFS | TFAP2 | TFAP2A | Transcription factor AP-2 | AP-2 transcription factor | AP2-alpha
Origin Species
Hu
NCBI GenBank
NM_003220
SwissProt
P05549

Protein

Expression System
HEK293T
Fusion Tag
FLAG, Myc
Molecular Weight
47.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSILAKMGDW QDRHDGTSNG TARLPQLGTV GQSPYTSAPP LSHTPNADFQ PPYFPPPYQP
61IYPQSQDPYS HVNDPYSLNP LHAQPQPQHP GWPGQRQSQE SGLLHTHRGL PHQLSGLDPR
121RDYRRHEDLL HGPHALSSGL GDLSIHSLPH AIEEVPHVED PGINIPDQTV IKKGPVSLSK
181SNSNAVSAIP INKDNLFGGV VNPNEVFCSV PGRLSLLSST SKYKVTVAEV QRRLSPPECL
241NASLLGGVLR RAKSKNGGRS LREKLDKIGL NLPAGRRKAA NVTLLTSLVE GEAVHLARDF
301GYVCETEFPA KAVAEFLNRQ HSDPNEQVTR KNMLLATKQI CKEFTDLLAQ DRSPLGNSRP
361NPILEPGIQS CLTHFNLISH GFGSPAVCAA VTALQNYLTE ALKAMDKMYL SNNPNSHTDN
421NAKSSDKEEK HRK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

433 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.