LSBio
TEV Protease Protein (Recombinant)

TEV Protease Protein (Recombinant)

ProteinLSBio Guarantee
Expression SystemEscherichia coli (Prokaryotic)
Format:Liquid
See all TEV Protease antibodies →
$259.00each
Catalog Number: LS-G27123-1000

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Overview

Description
Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin. Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by chromatographic techniques.
Category
Proteins & Peptides > Proteins > Recombinant Proteins

Protein

Expression System
Escherichia coli (Prokaryotic)
Molecular Weight
28.4 kDa (SDS-PAGE)
Protein Type
Recombinant

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
50 mM Tris, 5 mM DTT, 50% glycerol, pH 7.5.
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Store at -20°C, stable for 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.