LSBio
Human Rhinovirus 3C Protease Protein (Recombinant)

Human Rhinovirus 3C Protease Protein (Recombinant)

Expression SystemEscherichia coli (Prokaryotic)
Format:Liquid
$259.00each
Catalog Number: LS-G27166-100

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Overview

Description
Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro(LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant PreScission Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant PreScission Protease (rPSP) contains 217 amino acids with N-terminal His tag. A fully biologically active molecule, rPSP has a molecular mass of 24 kDa and is obtained by chromatographic techniques.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Origin Species
Virus / Viral Antigen

Protein

Expression System
Escherichia coli (Prokaryotic)
Molecular Weight
24 kDa (SDS-PAGE)
Protein Type
Recombinant

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 7.5.
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Store at -20°C, stable for 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.