Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TCEB3 / Elongin A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117494-100
Catalog Number: LS-G117494
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Elongin A(TCEB3) (NM_003198), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EloA | Elongin 110 kDa subunit | SIII | TCEB3 | TCEB3A | Elongin A | Elongin-A | SIII p110
- Origin Species
- Human
- NCBI GenBank
- NM_003198
- SwissProt
- Q14241
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAESALQVV EKLQARLAAN PDPKKLLKYL KKLSTLPITV DILAETGVGK TVNSLRKHEH61VGSFARDLVA QWKKLVPVER NAEPDEQDFE KSNSRKRPRD ALQKEEEMEG DYQETWKATG121SRSYSPDHRQ KKHRKLSELE RPHKVSHGHE RRDERKRCHR MSPTYSSDPE SSDYGHVQSP181PSCTSPHQMY VDHYRSLEED QEPIVSHQKP GKGHSNAFQD RLGASQERHL GEPHGKGVVS241QNKEHKSSHK DKRPVDAKSD EKASVVSREK SHKALSKEEN RRPPSGDNAR EKPPSSGVKK301EKDREGSSLK KKCLPPSEAA SDNHLKKPKH RDPEKAKLDK SKQGLDSFDT GKGAGDLLPK361VKEKGSNNLK TPEGKVKTNL DRKSLGSLPK VEETDMEDEF EQPTMSFESY LSYDQPRKKK421KKIVKTSATA LGDKGLKKND SKSTGKNLDS VQKLPKVNKT KSEKPAGADL AKLRKVPDVL481PVLPDLPLPA IQANYRPLPS LELISSFQPK RKAFSSPQEE EEAGFTGRRM NSKMQVYSGS541KCAYLPKMMT LHQQCIRVLK NNIDSIFEVG GVPYSVLEPV LERCTPDQLY RIEEYNHVLI601EETDQLWKVH CHRDFKEERP EEYESWREMY LRLQDAREQR LRVLTKNIQF AHANKPKGRQ661AKMAFVNSVA KPPRDVRRRQ EKFGTGGAAV PEKIKIKPAP YPMGSSHASA SSISFNPSPE721EPAYDGPSTS SAHLAPVVSS TVSYDPRKPT VKKIAPMMAK TIKAFKNRFS RRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
772 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.