LSBio
Human TCEB3 / Elongin A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human TCEB3 / Elongin A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G75710-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Elongin A(TCEB3) (NM_003198) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EloA | Elongin 110 kDa subunit | SIII | TCEB3 | TCEB3A | Elongin A | Elongin-A | SIII p110
Origin Species
Human
NCBI GenBank
NM_003198
SwissProt
Q14241

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAESALQVV EKLQARLAAN PDPKKLLKYL KKLSTLPITV DILAETGVGK TVNSLRKHEH
61VGSFARDLVA QWKKLVPVER NAEPDEQDFE KSNSRKRPRD ALQKEEEMEG DYQETWKATG
121SRSYSPDHRQ KKHRKLSELE RPHKVSHGHE RRDERKRCHR MSPTYSSDPE SSDYGHVQSP
181PSCTSPHQMY VDHYRSLEED QEPIVSHQKP GKGHSNAFQD RLGASQERHL GEPHGKGVVS
241QNKEHKSSHK DKRPVDAKSD EKASVVSREK SHKALSKEEN RRPPSGDNAR EKPPSSGVKK
301EKDREGSSLK KKCLPPSEAA SDNHLKKPKH RDPEKAKLDK SKQGLDSFDT GKGAGDLLPK
361VKEKGSNNLK TPEGKVKTNL DRKSLGSLPK VEETDMEDEF EQPTMSFESY LSYDQPRKKK
421KKIVKTSATA LGDKGLKKND SKSTGKNLDS VQKLPKVNKT KSEKPAGADL AKLRKVPDVL
481PVLPDLPLPA IQANYRPLPS LELISSFQPK RKAFSSPQEE EEAGFTGRRM NSKMQVYSGS
541KCAYLPKMMT LHQQCIRVLK NNIDSIFEVG GVPYSVLEPV LERCTPDQLY RIEEYNHVLI
601EETDQLWKVH CHRDFKEERP EEYESWREMY LRLQDAREQR LRVLTKNIQF AHANKPKGRQ
661AKMAFVNSVA KPPRDVRRRQ EKFGTGGAAV PEKIKIKPAP YPMGSSHASA SSISFNPSPE
721EPAYDGPSTS SAHLAPVVSS TVSYDPRKPT VKKIAPMMAK TIKAFKNRFS RR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

772 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.