Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBXT / T / Brachyury Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99988-100
Catalog Number: LS-G99988
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human T(T) (NM_003181), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brachyury | Brachyury protein | Protein T | TFT | T | T brachyury (mouse) homolog | T brachyury homolog | brachyury homolog (mouse) | TBXT
- NCBI GenBank
- NM_003181
- SwissProt
- O15178
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSPGTESAG KSLQYRVDHL LSAVENELQA GSEKGDPTER ELRVGLEESE LWLRFKELTN61EMIVTKNGRR MFPVLKVNVS GLDPNAMYSF LLDFVAADNH RWKYVNGEWV PGGKPEPQAP121SCVYIHPDSP NFGAHWMKAP VSFSKVKLTN KLNGGGQIML NSLHKYEPRI HIVRVGGPQR181MITSHCFPET QFIAVTAYQN EEITALKIKY NPFAKAFLDA KERSDHKEMM EEPGDSQQPG241YSQSGGWLLP GTSTLCPPAN PHPQFGGALS LPSTHSCDRY PTLRSHRSSP YPSPYAHRNN301SPTYSDNSPA CLSMLQSHDN WSSLGMPAHP SMLPVSHNAS PPTSSSQYPS LWSVSNGAVT361PGSQAAAVSN GLGAQFFRGS PAHYTPLTHP VSAPSSSGSP LYEGAAAATD IVDSQYDAAA421QGRLIASWTP VSPPSMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
436 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.