PathPlus™
PathPlus™ TBXT / T / Brachyury Antibody (clone RBT-TBXT)
Catalog Number: LS-B16823-0.05
0.05 mL / $470.00
Antibody: Rabbit Recombinant Monoclonal
Applications: Immunohistochemistry (general)
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human TBXT / T / Brachyury Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G85303-20
Catalog Number: LS-G85303
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human T(T) (NM_003181) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brachyury | Brachyury protein | Protein T | TFT | T | T brachyury (mouse) homolog | T brachyury homolog | brachyury homolog (mouse) | TBXT
- Origin Species
- Human
- NCBI GenBank
- NM_003181
- SwissProt
- O15178
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSPGTESAG KSLQYRVDHL LSAVENELQA GSEKGDPTER ELRVGLEESE LWLRFKELTN61EMIVTKNGRR MFPVLKVNVS GLDPNAMYSF LLDFVAADNH RWKYVNGEWV PGGKPEPQAP121SCVYIHPDSP NFGAHWMKAP VSFSKVKLTN KLNGGGQIML NSLHKYEPRI HIVRVGGPQR181MITSHCFPET QFIAVTAYQN EEITALKIKY NPFAKAFLDA KERSDHKEMM EEPGDSQQPG241YSQSGGWLLP GTSTLCPPAN PHPQFGGALS LPSTHSCDRY PTLRSHRSSP YPSPYAHRNN301SPTYSDNSPA CLSMLQSHDN WSSLGMPAHP SMLPVSHNAS PPTSSSQYPS LWSVSNGAVT361PGSQAAAVSN GLGAQFFRGS PAHYTPLTHP VSAPSSSGSP LYEGAAAATD IVDSQYDAAA421QGRLIASWTP VSPPSMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
436 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
