Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBCK / TBCKL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128388-100
Catalog Number: LS-G128388
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HSPC302(TBCK) (NM_001163436), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TBCK | TBCKL | TBC1 domain containing kinase | HSPC302
- Origin Species
- Human
- NCBI GenBank
- NM_033115
- SwissProt
- Q8TEA7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFPLKDAEMG AFTFFASALP HDVCGSNGLP LTPNSIKILG RFQILKTITH PRLCQYVDIS61RGKHERLVVV AEHCERSLED LLRERKPVSC STVLCIAFEV LQGLQYMNKH GIVHRALSPH121NILLDRKGHI KLAKFGLYHM TAHGDDVDFP IGYPSYLAPE VIAQGIFKTT DHMPSKKPLP181SGPKSDVWSL GIILFELCVG RKLFQSLDIS ERLKFLLTLD CVDDTLIVLA EEHGCLDIIK241ELPETVIDLL NKCLTFHPSK RPTPDELMKD KVFSEVSPLY TPFTKPASLF SSSLRCADLT301LPEDISQLCK DINNDYLAER SIEEVYYLWC LAGGDLEKEL VNKEIIRSKP PICTLPNFLF361EDGESFGQGR DRSSLLDDTT VTLSLCQLRN RLKDVGGEAF YPLLEDDQSN LPHSNSNNEL421SAAATLPLII REKDTEYQLN RIILFDRLLK AYPYKKNQIW KEARVDIPPL MRGLTWAALL481GVEGAIHAKY DAIDKDTPIP TDRQIEVDIP RCHQYDELLS SPEGHAKFRR VLKAWVVSHP541DLVYWQGLDS LCAPFLYLNF NNEALAYACM SAFIPKYLYN FFLKDNSHVI QEYLTVFSQM601IAFHDPELSN HLNEIGFIPD LYAIPWFLTM FTHVFPLHKI FHLWDTLLLG NSSFPFCIGV661AILQQLRDRL LANGFNECIL LFSDLPEIDI ERCVRESINL FCWTPKSATY RQHAQPPKPS721SDSSGGRSSA PYFSAECPDP PKTDLSRESI PLNDLKSEVS PRISAEDLID LCELTVTGHF781KTPSKKTKSS KPKLLVVDIR NSEDFIRGHI SGSINIPFSA AFTAEGELTQ GPYTAMLQNF841KGKVIVIVGH VAKHTAEFAA HLVKMKYPRI CILDGGINKI KPTGLLTIPS PQITRTRPLEQKLISEEDLAANDILDYKDDDDKV
893 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.